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Living Modified Organism
(LMO)
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
BioCassava Pro-vitamin A cassava
EN
DXS+PSY
No
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Nairobi,Organization:Kenya Agricultural & Livestock Research Organisation (KALRO)Government agency (National/Federal)
, KenyaPhone: +254 418330120,Fax:Email: directorgeneral@kalro.org,Website:
The cassava (Manihot esculenta) was modified for increased pro-vitamin A (trans beta-carotene) content through the expression of Pantoea ananas phytoene desaturase and Arabidopsis thaliana 1-deoxy-D-xylulose-5-phosphate synthase. Phytoene synthase directs the flux of geranylgeranyl diphosphate into carotenoid biosynthesis, while 1-deoxy-D-xylulose-5-phosphate synthase, which is a rate limiting step in the overall pathway, catalyzes the first step in isoprenoid and carotenoid biosynthesis. Overall, up-regulating the total flux in the isoprenoid pathway and to enhance the flux of isoprenoids into carotenoid synthesis results in a high accumulation of provitamin A in tuber tissues. The modified cassava also expresses Escherichia coli neomycin phosphotransferase II, which was a kanamycin or neomycin selectable marker during transformation.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-112539-1 Organism Manihot esculenta (Cassava, Brazilian arrowroot, Yuca, Manioc, Mandioca, MANES)Crops
Variety 60444
EN
pILTAB505
EN
- Agrobacterium-mediated DNA transfer
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-100273-4 B33 gene promotor | Solanum tuberosum (Potato, SOLTU)Promoter
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BCH-GENE-SCBD-263476-1 Δ4 acyl-ACP desaturase transit peptide | Coriandrum sativum (Coriander, Cilantro, Dhania, Chinese parsley)Transit signal
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BCH-GENE-SCBD-103621-3 Phytoene Desaturase gene | Erwinia uredovora (PANAN)Protein coding sequence | Changes in quality and/or metabolite content (Vitamins)
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BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminator
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BCH-GENE-SCBD-263474-2 1-deoxy-D-xylulose-5-phosphate synthase | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)Protein coding sequence | Changes in quality and/or metabolite content (Vitamins)
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BCH-GENE-SCBD-100366-6 CaMV Enhanced 35S promoter | Cauliflower mosaic virus (CaMV)Promoter
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BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)Protein coding sequence | Resistance to antibiotics (Kanamycin)
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BCH-GENE-SCBD-100290-6 CaMV 35S terminator | Cauliflower mosaic virus (CaMV)Terminator
The modified cassava contains three gene cassettes: Pantoea ananas phytoene desaturase (crtI), Arabidopsis thaliana 1-deoxy-D-xylulose-5-phosphate synthase (dxs) and Escherichia coli neomycin phosphotransferase II (nptII).
The crtI coding sequence is under control of a potato (Solanum tuberosum) patatin-1 (B33) promoter and an Agrobacterium tumefaciens nopaline synthase (nos) terminator. An N-terminal Coriandrum sativum palmitoyl-[acyl-carrier-protein] 4-desaturase transit peptide was added to localize the translated protein to the plastids. Due to the nature of the promoter, transcription from this gene cassette is expected to be restricted to the tuber and root tissues of the plant.
The dxs sequence is under control of a B33 promoter and a nos terminator. Due to the nature of the promoter, transcription from this gene cassette is expected to be restricted to the tuber and root tissues of the plant.
The nptII sequence is under control of a Cauliflower mosaic virus enhanced promoter and terminator. Due to the constitutive nature and duplicated enhancers, transcription is expected to occur at high levels in all plant tissues.
The crtI coding sequence is under control of a potato (Solanum tuberosum) patatin-1 (B33) promoter and an Agrobacterium tumefaciens nopaline synthase (nos) terminator. An N-terminal Coriandrum sativum palmitoyl-[acyl-carrier-protein] 4-desaturase transit peptide was added to localize the translated protein to the plastids. Due to the nature of the promoter, transcription from this gene cassette is expected to be restricted to the tuber and root tissues of the plant.
The dxs sequence is under control of a B33 promoter and a nos terminator. Due to the nature of the promoter, transcription from this gene cassette is expected to be restricted to the tuber and root tissues of the plant.
The nptII sequence is under control of a Cauliflower mosaic virus enhanced promoter and terminator. Due to the constitutive nature and duplicated enhancers, transcription is expected to occur at high levels in all plant tissues.
EN
- Food
EN
Note: At the time of publication, the modified cassava was still in research and development. Thus, a specific transformation event will be designated after a line has been chosen for later development and/or commercialization.
- Gene expression of beta carotene genes in transgenic biofortified cassava.pdf [ English ]
- Opportunities for biofortification of cassava for sub-Saharan Africa - the biocassava plus program.pdf [ English ]
- Provitamin A biofortification of cassava enhances shelf life but reduces dry.pdf [ English ]
- Popular Biofortified Cassava Cultivars Are Heavily Impacted by Plant Parasitic Nematodes, Especially Meloidogyne Spp.pdf [ English ]
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