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Living Modified Organism
(LMO)
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Pod borer-resistant cowpea
EN
245F
Yes
AAT-Ø245F-3
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Person:AATF and Institute of Agricultural ResearchARCN Annex No. 3 Ibrahim Idris Street, Jabi( AATF) Institute of Agricultural Research, Ahmadu Bello University, P.M.B 1044, Zaria, Nigeria.Abuja, F.C.T
, NigeriaPhone: 254-204223700, +234 8028373464,Fax: 254-204223701,Email: rsadamu@gmail.com,Website:Related OrganizationAfrican Agricultural Technology Foundation and Institute of Agricultural Research ()Academic or research instituteARCN Annex No. 3 Ibrahim Idris Street, Jabi( AATF) Institute of Agricultural Research, Ahmadu Bello University, P.M.B 1044, Zaria, Nigeria.Abuja, F.C.T
, NigeriaPhone: 254-204223700, +234 8028373464,Fax: 254-204223701,Email: rsadamu@gmail.com,Website:
Cowpea (Vigna unguiculata) was modified through Agrobacterium-mediate transformation to confer resistance to Lepidoptera pests, particularly pod borer (Maruca vitrata). The modified cowpea expresses Bacillus thuringiensis crystal protein Cry2Ab2, which has a pore-forming mode of action that selectively damages the midgut epithelium line of feeding larvae, resulting in cell lysis and septicemia and leading to death of the feeding larvae. In addition, the modified cowpea expresses a Escherichia coli neomycin phosphotransferase II cassette, which functioned as a selectable marker for kanamycin resistance during transformation of the cowpea line.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-103617-3 Organism Vigna unguiculata (Cowpea, Black eyed pea)Crops
Cultivar IT86D-1010
EN
pMB6
EN
- Agrobacterium-mediated DNA transfer
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1.726 kb
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0.273 kb
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1.905 kb
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0.373 kb
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0.523 kb
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0.117 kb
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0.119 kb
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0.618 kb
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0.139 kb
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-103851-5 rbcS Promoter | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)Promoter
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BCH-GENE-SCBD-14988-7 Cry2Ab2 | Bacillus thuringiensis (Bt, Bacillus, BACTU)Protein coding sequence | Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths))
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BCH-GENE-SCBD-103853-2 rbcS Terminator | Nicotiana tabacum (Tobacco, TOBAC )Terminator
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BCH-GENE-SCBD-114438-3 SCSV S1 promoter | Subterranean clover stunt virus (SCSV, Subterranean clover stunt virus)Promoter
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BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)Protein coding sequence | Resistance to antibiotics (Kanamycin)
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BCH-GENE-SCBD-114440-2 SCSV3 terminator | Subterranean clover stunt virus (SCSV, Subterranean clover stunt virus)Terminator
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BCH-GENE-SCBD-101902-5 Ribulose-1,5-bisphosphate carboxylase small subunit transit peptide | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)Transit signal
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BCH-GENE-SCBD-114274-2 Catalase 1 intron | Ricinus communis (Castor bean)Intron
The modified cowpea expresses two gene cassettes: Bacillus thuringiensis cry2Ab2 and Escherichia coli neomycin phosphotransferase II (nptII).
Transcription of cry2ab2 is controlled by an Arabidopsis thaliana ribulose-1,5-bisphosphate carboxylase small subunit promoter and Nicotiana tabacum ribulose-1,5-bisphosphate carboxylase small subunit terminator. The transit peptide of the ribulose-1,5-bisphosphate carboxylase small subunit coding sequence has also been included to direct the translated peptide to the chloroplasts.
Transcription of nptII is controlled by a Subterranean clover stunt virus DNA segment 1 promoter and DNA segment 3 terminator. The coding sequence of nptII is interrupted by the presence of a Ricinus communis catalase 1 intron to prevent leaky expression of the nptII gene cassette. Due to the nature of the promoter, elevated levels of transcription of nptII are expected from this gene cassette.
Note:
Transcription of cry2ab2 is controlled by an Arabidopsis thaliana ribulose-1,5-bisphosphate carboxylase small subunit promoter and Nicotiana tabacum ribulose-1,5-bisphosphate carboxylase small subunit terminator. The transit peptide of the ribulose-1,5-bisphosphate carboxylase small subunit coding sequence has also been included to direct the translated peptide to the chloroplasts.
Transcription of nptII is controlled by a Subterranean clover stunt virus DNA segment 1 promoter and DNA segment 3 terminator. The coding sequence of nptII is interrupted by the presence of a Ricinus communis catalase 1 intron to prevent leaky expression of the nptII gene cassette. Due to the nature of the promoter, elevated levels of transcription of nptII are expected from this gene cassette.
Note:
- Sequencing analysis indicated that a single insertion of the T-DNA was present in the parental genome and 41-basepair and 26-basepair truncations at the 5’ and 3’ termini, respectively, occurred. Further, analysis of 5’ and 3’ flanking host genomic DNA, the site of T-DNA insertion was mapped to an intergenic region on chromosome 1, and there were no new novel junction-spanning open reading frames created as a consequence of the genetic transformation.
EN
- Food
EN
EN
| Record type | Field | Record(s) | |
|---|---|---|---|
| Risk Assessment generated by a regulatory process | Living modified organism(s) | 2 | |
| Country's Decision or any other Communication | LMO identification | 1 | |
| Living Modified Organism | Recipient Organism or Parental Organisms | 1 | |
