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Living Modified Organism
(LMO)
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Biotech Vac Salmonella
EN
Biotech Vac Salmonella
No
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Organization:Vetanco do Brasil Importação e Exportação Ltda (Vetanco)R. Edgar Marchiori, 255 - Distrito Industrial Benedito Storani, Vinhedo - SP, 13280-000Vinhedo - SP,
, BrazilPhone: [49] 9 9119.1020,Fax:Email: contato@vetanco.com.br,Website: https://www.vetanco.com/br/,
Biotech Vac Salmonella is an oral recombinant veterinary vaccine intended to induce protective immunity against Salmonella caused by different species, including Salmonella enteritidis and Salmonella infantis. To achieve protection against Salmonella serotypes, B. subtilis expresses the TolB protein subunit which acts as the vaccine antigen and induces an immune response that contributes to protection the disease. The final vaccine contains no viable modified bacteria, as the recombinant B. subtilis is inactivated before formulation.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-103064-6 Organism Bacillus subtilis (Bacillus, BACIU)Bacteria
Bacillus subtilis 1A6 (ATCC® 39085TM)
EN
pHT01
EN
- Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-105183-2 Phosphoserine aminotransferase gene | Salmonella typhimurium (SALTM)Protein coding sequence | Changes in quality and/or metabolite content (Protein and amino acids)
Information on the inserted DNA sequence
The modified Bacillus subtilis contains a recombinant pHT01 plasmid carrying a genetic expression cassette for the expression of the TolB antigen from Salmonella enterica subsp. enterica serovar Typhimurium strain LT2 used in the vaccine formulation. The DNA sequence encoding the Salmonella TolB protein was cloned into the pHT01 expression plasmid. The coding sequence was inserted into the BamHI/XbaI multiple cloning site of the expression plasmid.
Expression of the TolB coding sequence is regulated by a LacI/PgroESL regulatory cassette, in which the groESL operon is composed of the groEL chaperonin and groES helper protein from Bacillus subtilis and transcription is controlled by the LacI repressor encoded by the lacI gene and induced by isopropyl β-D-1-thiogalactopyranoside (IPTG). Production of the TolB protein is inactivated by treatment with formalin before being used as a vaccine. The final product is intended to be orally administered to poultry.
The modified Bacillus subtilis contains a recombinant pHT01 plasmid carrying a genetic expression cassette for the expression of the TolB antigen from Salmonella enterica subsp. enterica serovar Typhimurium strain LT2 used in the vaccine formulation. The DNA sequence encoding the Salmonella TolB protein was cloned into the pHT01 expression plasmid. The coding sequence was inserted into the BamHI/XbaI multiple cloning site of the expression plasmid.
Expression of the TolB coding sequence is regulated by a LacI/PgroESL regulatory cassette, in which the groESL operon is composed of the groEL chaperonin and groES helper protein from Bacillus subtilis and transcription is controlled by the LacI repressor encoded by the lacI gene and induced by isopropyl β-D-1-thiogalactopyranoside (IPTG). Production of the TolB protein is inactivated by treatment with formalin before being used as a vaccine. The final product is intended to be orally administered to poultry.
Note
- In order to confirm the construct, the recombinant pHT01 plasmid containing the cloned Salmonella TolB protein gene was first inserted into Escherichia coli TOPO 1 by direct ligation. After confirmation, it was inserted then into Bacillus subtilis 1A6 (ATCC® 39085TM) with the same method.
- The vector pHT01 is made up of 7,956 bp where the DNA sequence encoding the Salmonella TolB protein (tolB gene) is composed of 1,293 bp.
- Polymerase chain reaction, with primers that amplify a 1,346 bp fragment, is used to detect the presence of the recombinant plasmid in Escherichia coli and B. subtilis.
- For selection of positive transformants in Escherichia coli and B. subtilis, pHT01 contains selection markers for antibiotics resistance genes, ampicillin and chloramphenicol.
EN
- Vaccine
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- Biotech Vac Salmonella is an inactivated oral subunit vaccine containing a modified derivative produced by Bacillus subtilis. The LM is inactivated prior to incorporation into the final vaccine formulation and is not intended to replicate following administration.
- The transformation event listed is the transformation event provided by the submitter. Should there be another transformation event available or additional information regarding the modification of Bacillus subtilis for the use of vaccine antigen against different Salmonella serotypes, the record will be updated.
- CTNBio Technical Summary: Request for Approval of Commercial Release of the product BIOTECH VAC SALMONELLA [PDF] ( Ministério da Ciência, Tecnologia, Inovações e Comunicações (MCTI); Solicitação de Aprovação de Liberação Comercial; Parecer Técnico; Comissão técnica nacional de biossegurança; National Technical Commission on Biosafety ) [ Portuguese ]
- Vetanco: Evaluation of long term immunity and protection against Salmonella spp by orally administrated inactivated vaccine [URL] ( International Poultry Science Forum (IPPE) 2018; BV Science Poster ) [ English ]
- Vetanco and Instituto de Investigación Patobiología: Protection conferred by a subunit Salmonella vaccine against Salmonella infantis in broiler chickens [URL] ( Centro de Investigación en Ciencias Veterinarias y Agronómicas (CICVyA); Instituto Nacional de Tecnología Agropecuaria (INTA); International Poultry Science Forum (IPPE) 2018; BV Science Poster ) [ English ]
