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Living Modified Organism (LMO)
  |  
Decisions on the LMO Risk Assessments  
published: 16 Aug 2007 last updated: 06 Sep 2012
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Non flowering apple tree, modified for resistance to fungi
EN
Multiple events
No
The genetically modified apple trees are inoculated onto non-modified rootstocks. The GM apples carry a kanamycin resistance gene nptII, a modified beta-glucuronidase gene (GUS-intron) and a hth gene encoding a type 1 alpha-hordothionine precursor conferring resistance to fungi.
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
EN
Characteristics of the modification process
pMOG402.hth.gusintron
EN
  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)
    Protein coding sequence | Resistance to antibiotics (Kanamycin)
  • BCH-GENE-SCBD-46091-2 Alpha-hordothionine gene | Hordeum vulgare (Barley, HORVU)
    Protein coding sequence | Resistance to diseases and pests (Fungi)
  • BCH-GENE-SCBD-46004-7 Beta-glucuronidase coding sequence | Escherichia coli (ECOLX)
    Protein coding sequence | Selectable marker genes and reporter genes
  • BCH-GENE-SCBD-100270-6 Nopaline Synthase Gene Promoter | Agrobacterium tumefaciens (Agrobacterium)
    Promoter
  • BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)
    Terminator
  • BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)
    Promoter
  • BCH-GENE-SCBD-100290-6 CaMV 35S terminator | Cauliflower mosaic virus (CaMV)
    Terminator
Additional information on the inserted DNA sequences
The binary vector pMOG402.hth.gusintron contains between the borders the following elements:
- gus(int): B-glucuronidase gene, besides original version also a version with an intron inserted which originates from plants

Vector information
The binary vector pMOG402.hth.gusintron is a derived from pBIN19. It can be propagated both in E. coli as well as A. tumefaciens.
EN
LMO characteristics
EN
  • Food
Detection method(s)
EN
Additional Information
EN
Records referencing this document Show in search
Record type Field Record(s)
Living Modified Organism Related LMO(s) 1
Risk Assessment generated by a regulatory process Living modified organism(s) 1