Canarypox virus (CNPV) is an avipoxvirus and etiologic agent of canarypox, a disease of birds both in the wild and in commercial aviaries, where significant losses result.

CNPV has been successfully used as a host range-restricted mammalian expression vector and is a vaccine vector of increasing importance, with CNPV-based veterinary vaccines commercially available and human vaccines undergoing clinical trials. Licensed and experimental CNPV-based vaccines, most of which utilize the highly attenuated ALVAC strain of CNPV, encode a range of pathogen and tumor-associated antigens, including those from rabies virus, canine distemper virus, feline leukemia virus, human immunodeficiency virus, human cytomegalovirus, hepatitis B and C viruses, Plasmodium falciparum, melanoma, and colorectal cancer.