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Living Modified Organism
(LMO)
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Cisgenic apple trees with scab resistance
EN
HcrVf2
No
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Person:Plant Research International, Dept. Genetics and BreedingPO Box 59Wageningen,
6700 AB, Netherlands (Kingdom of the)Phone: +31 (0)317 48 36 80,Fax:Email: info@wur.nl,Website: http://www.wur.nl/uk/,Related OrganizationPlant Research International (PRI)Academic or research institutePO Box 59Wageningen,
6700 AB, Netherlands (Kingdom of the)Phone: +31 (0)317 48 36 80,Fax:Email: info@wur.nl,Website: http://www.wur.nl/uk/,
Resistance to the fungus Venturia inaequalis, causative agent of the disease called apple scab, is the trait that was introduced. No further marker or other genes are present.
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-12099-5 Organism Malus domestica (Apple, MALDO)Crops
EN
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Non flowering apple tree, modified for resistance to fungi| Plant Research International | Resistance to antibiotics (Kanamycin), Resistance to diseases and pests (Fungi), Selectable marker genes and reporter genes
pMF1
EN
- Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-103738-1 Homologues of Cladosporium fulvum Resistance genes of the Vf region | Malus domestica (Apple, MALDO)Protein coding sequence | Resistance to diseases and pests (Fungi)
The recombinase located on the pMF1 backbone can be activated by adding dexamethason resulting in the excision of the vector backbone. As a result of this excision only the intended trait will be present in the LMO.
Three distinct constructs were generated:
SPVf2: RB --> Native HcrVf2 gene promoter --> Hcr2Vf2 coding region --> Native HcrVf2 gene terminator --> LB
CH12: RB --> Native HcrVf2 gene promoter --> Hcr2Vf2 coding region --> Native HcrVf2 gene terminator --> LB
RbcVf2: RB --> Apple Rubisco promoter --> HcrVf2 --> Apple Rubisco Terminator --> LB
EN
Three distinct constructs were generated:
SPVf2: RB --> Native HcrVf2 gene promoter --> Hcr2Vf2 coding region --> Native HcrVf2 gene terminator --> LB
CH12: RB --> Native HcrVf2 gene promoter --> Hcr2Vf2 coding region --> Native HcrVf2 gene terminator --> LB
RbcVf2: RB --> Apple Rubisco promoter --> HcrVf2 --> Apple Rubisco Terminator --> LB
EN
- Research
EN
EN
| Record type | Field | Record(s) | |
|---|---|---|---|
| Risk Assessment generated by a regulatory process | Living modified organism(s) | 1 | |
