NMK-89576-1 - Superior NewLeaf™ potato | BCH-LMO-SCBD-14900 | Living Modified Organism | Biosafety Clearing-House

Living Modified Organism (LMO)
Decisions on the LMO Risk Assessments  
published: 05 Jun 2006 last updated: 26 Apr 2013
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Superior NewLeaf™ potato
The transgenic potatoes were genetically engineered to be resistant to attack by Colorado potato beetle  by producing their own insecticide. These lines were developed by introducing the cry3A gene.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
Cultivar: Superior
  • NMK-89724-5 - Superior NewLeaf™ potato
    | Monsanto | Resistance to antibiotics (Kanamycin), Resistance to diseases and pests (Insects, Coleoptera (beetles))
Characteristics of the modification process
  • Agrobacterium-mediated DNA transfer
0.620 kb
1.800 kb
0.360 kb
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
The coding sequence of the Cry3A gene was modified to plant preferred codons. This resulted in changes to 399 of 1791 nucleotides but there were no changes to the resulting amino acid sequence.

Southern blot analysis indicated that they Cry3A gene and regulatory elements were incorporated into the host genome. The transformation cassette also contained an nptII gene however this was not transferred into the genome. Additionally segments of the ori-v and ori322 coding sequences, which ere outside the left and right borders, were detected.
LMO characteristics
  • Food
  • Feed
Detection method(s)
Additional Information
The Cry3A protein expressed in these transgenic potato cultivars is identical to that found in nature and in commercial Bt spray formulations. Cry proteins, of which Cry3A is only one, act by selectively binding to specific sites localized on the lining of the midgut of susceptible insect species. Following binding, pores are formed that disrupt midgut ion flow causing gut paralysis and eventual death due to bacterial sepsis. Cry3A is insecticidal only when eaten by the larvae of coleopteran insects such as Colorado potato beetle and its specificity of action is directly attributable to the presence of specific binding sites in the target insects. There are no binding sites for delta-endotoxins of B. thuringiensis on the surface of mammalian intestinal cells, therefore, livestock animals and humans are not susceptible to these proteins.