Barnase | BCH-GENE-SCBD-14973 | Genetic element | Biosafety Clearing-House

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Genetic element (GENE)

last updated: 23 Feb 2021
General information
Barnase
EN
CS-barnase-BACAM
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Protein coding sequence
No
Donor organism
EN
Characteristics of the protein coding sequence
Barnase ribonuclease inhibitor
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Barnase is a bacterial protein that has ribonuclease activity. It is lethal to the cell when expressed without its inhibitor barstar. The inhibitor binds to and occludes the ribonuclease active site, preventing barnase from damaging the cell's RNA after it has been synthesized but before it has been secreted.

Barnase catalyzes hydrolysis at diribonucleotide GpN sites. Cleavage occurs in two steps using a general acid-base mechanism: a cyclic intermediate is formed during the first transesterification step, which is then hydrolysed to release the cleaved RNA.

When synthesised by plants it causes male sterility by producing a ribonuclease enzyme (RNAse) that interferes with RNA production in specific cells of the pollen sac, thus disrupting their normal cell functioning and preventing the development of pollen which lead to male sterility.
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Additional Information
EN
Records referencing this document Show in search
Record type Field Record(s)
Living Modified Organism Introduced or modified genetic element(s) 22
Living Modified Organism Genetic elements construct 22
Laboratory for detection and identification of LMOs Genetic element(s) detectable by the laboratory 2