Oilseed rape contains anti-nutrient components, phytic acid, sinapine and tannins. Phytic acid chelates mineral nutrients, including calcium, magnesium, potassium, iron, and zinc, rendering them biologically unavailable to mono-gastric animals consuming the seed. Sinapine imparts a bitter taste and reduces palatability of the seed for some animal species. Tannins are a third anti-nutrient found in canola seed for some animal species.  Although tannins have been shown to have anti-oxidant properties, they also chelate metals such as iron and zinc reducing the absorption of these nutrients. In the combined-site analysis, no significant difference (= 0.05) was observed between MON 88302 and the conventional control for indolylglucosinolates, total glucosinolates, phytic acid, sinapine and tannins. The statistical analyses found a combined-site significant difference in alkyl glucosinolates that was lower than the conventional mean value. The mean alkyl glucosinolates value for MON 88302 was within the natural variability of commercial canola defined by the 99% tolerance interval established from the concurrently grown commercial reference varieties with a history of safe consumption, and the value was within the safety threshold for canola. Total glucosinolate levels in seed from MON 88302 was  within the standard for canola. Thus, an evaluation of anti-nutrient components in seed supports the conclusion that MON 88302 is as safe as and compositionally equivalent to conventional canola.