SYN-IR162-4 - Agrisure™ Viptera maize | BCH-LMO-SCBD-100885 | Living Modified Organism | Biosafety Clearing-House

Loading...
  |  

Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
last updated: 05 Jun 2017
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Agrisure™ Viptera maize
EN
MIR162
Yes
SYN-IR162-4
MIR162 maize is transformed with vip3Aa20 gene which encodes the Vip3Aa20 protein that confers resistance against lepidopteran insect pests  including: fall armyworm, armyworm , beet armyworm , corn earworm , black cutworm , and western bean cutworm. Event MIR162 maize also contains the manA gene from Escherichia coli, which encodes the selectable marker, phosphomannose isomerase (PMI).

Vip3A is a group of vegetative insecticidal proteins (i.e., produced during the vegetative stage of bacterial growth) from Bacillus thuringiensis. The vip3Aa20 form inserted into MIR162 is a variant of the native vip3Aa gene, which was isolated from Bt strain AB88.
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
EN
Characteristics of the modification process
pNOV1300
EN
  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-100887-5 Vegetative insecticidal protein 3Aa20 | Bacillus thuringiensis (Bt, Bacillus, BACTU)
    Protein coding sequence | Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths))
  • BCH-GENE-SCBD-15003-7 Phosphomannose Isomerase gene | Escherichia coli (ECOLX)
    Protein coding sequence | Mannose tolerance,Selectable marker genes and reporter genes
  • BCH-GENE-SCBD-100362-7 Ubiquitin gene promoter | Zea mays (Maize, Corn, MAIZE)
    Promoter
  • BCH-GENE-SCBD-100290-6 CaMV 35S terminator | Cauliflower mosaic virus (CaMV)
    Terminator
  • BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)
    Terminator
  • BCH-GENE-SCBD-101406-4 Phosphoenolpyruvate carboxylase, intron 9 | Zea mays (Maize, Corn, MAIZE)
    Intron
In MIR162 maize, a variant of the native Bt Vip3Aa, named vip3Aa19, which has codon changes that result in a single  M129I amino acid substitution was inserted into the transformation cassette. During the transformation process an additional DNA mutation resulted in a K284Q amino acid substitution. This final form was designated the name Vip3Aa20.

The pNOV1300 vector region between the left and right border sequences, which included the vip3Aa19 and pmi gene expression cassettes, was inserted into the maize genome during transformation.

Southern blot analyses demonstrated that the T-DNA insert contains: i) single copies of a vip3Aa20 gene and a pmi gene; ii) two copies of the ZmUbiInt promoter; iii) one copy of the NOS terminator; and iv) no backbone sequences from transformation plasmid pNOV1300.
EN
LMO characteristics
EN
  • Food
  • Feed
Additional Information
EN
Records referencing this document Show in search
Record type Field Record(s)
Loading...