BPS-CV127-9 - Herbicide-tolerant soybean | BCH-LMO-SCBD-100994 | Living Modified Organism | Biosafety Clearing-House


Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
last updated: 15 Feb 2013
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Herbicide-tolerant soybean
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
Characteristics of the modification process
6.2 kb linear fragment PvuII fragment derived from plasmid pAC321
  • Biolistic / Particle gun
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-48073-8 Acetohydroxy acid synthase gene | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)
    Protein coding sequence | Resistance to herbicides (Imidazolinone, Sulfonylurea)
The csr1-2 coding sequence from Arabisopsis thaliana is 2013 bp long and includes the S653N point mutation which confers tolerance to imidazolinone herbicides.

In CV127, transcription is directed by the 5’ and 3’ untranslated regions (UTR) containing the putative promoter and terminator regions, respectively, of the csr1-2 gene from Arabidopsis thaliana.

In addition to the S653N mutation found in the donor organism, DNA sequence analysis revealed that the csr1-2 gene cassette contains three point mutations relative to the PvuII linear DNA fragment of pAC321. One of the point mutations is a G to A mutation at position 272 in the csr1-2 gene, which results in an amino acid change from arginine to lysine. The other two mutations are genetically silent.

Sequence analysis revealed that parts of the PvuII transformation fragment are not contained within the transgene insert in CV127. Deletions of unannotated Arabidopsis genomic DNA occurred both at the 5’ end and 3’ end during insertion into the soybean genome.
LMO characteristics
  • Food
  • Feed
Detection method(s)
Additional Information
Description of the methods used for the genetic modification

A purified, linear DNA fragment derived from plasmid pAC321 was used to
transform embryogenic axis tissue derived from the apical meristem of a single soybean seed of the commercial variety Conquista using particle bombardment. No carrier DNA was used in the process.

Nature and source of the vector used
Soybean tissues were transformed with an approximately 6.2 kb linear fragment PvuII fragment derived from plasmid pAC321 containing the csr1-2 gene cassette.