MON-877Ø5-6 - Vistive Gold™ Soybean | BCH-LMO-SCBD-104683 | Living Modified Organism | Biosafety Clearing-House


Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
last updated: 22 Jan 2014
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Vistive Gold™ Soybean
MON 87705
The modified fatty acid profile in MON 87705 soybean oil is achieved through the use of endogenous soybean (Glycine max L.) gene segments configured to suppress FATB and FAD2 gene expression.  MON 87705 contains FATB1-A and FAD2-1A gene segments under the control of a seed promoter, limiting oil composition modification to this tissue. 

The assembled gene transcript has an inverted repeat that produces double stranded RNA (dsRNA) that, via the RNA interference (RNAi) pathway, suppresses endogenous FATB and  FAD2  gene expression, thereby producing the  desired fatty acid phenotype
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
Characteristics of the modification process
  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-14979-7 5-enolpyruvylshikimate-3-phosphate synthase gene | Agrobacterium tumefaciens (Agrobacterium)
    Protein coding sequence | Resistance to herbicides (Glyphosate)
  • BCH-GENE-SCBD-100267-2 delta(12)-fatty acid dehydrogenase | Glycine max (Soybean, Soya bean, Soya, SOYBN)
    Protein coding sequence | Changes in quality and/or metabolite content (Lipid and fatty acids)
  • BCH-GENE-SCBD-104682-2 Palmitoyl acyl carrier protein thioesterase 5'UTR | Glycine max (Soybean, Soya bean, Soya, SOYBN)
  • BCH-GENE-SCBD-100365-6 Chloroplast transit peptide 2 | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)
    Transit signal
  • BCH-GENE-SCBD-101877-5 rbcS-E9 gene terminator | Pisum sativum (Garden pea, PEA)
  • BCH-GENE-SCBD-104359-2 α' subunit of β-conglycinin gene promoter | Glycine max (Soybean, Soya bean, Soya, SOYBN)
  • BCH-GENE-SCBD-104689-2 H6 gene 3'UTR | Gossypium barbadense (Sea-island cotton, Egyptian cotton, GOSBA)
  • BCH-GENE-SCBD-103903-1 Elongation factor EF-1alpha promoter | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)
  • BCH-GENE-SCBD-103904-1 Elongation factor EF-1alpha Leader | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)
  • BCH-GENE-SCBD-103905-1 Elongation factor EF-1alpha Intron 1 | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)
  • BCH-GENE-SCBD-105196-2 FMV 35S Enhancer | Figwort mosaic virus (Figwort mottle virus, FMV, CMoVb)
The PV-GMPQ/HT4404 vector contains two T-DNAs, each delineated by left and right border regions. 

T-DNA I contains a a codon optimised cp4 epsps expression cassette and a partial suppression cassette. The  cp4 epsps expression cassette is under the regulation of FMV/Tsf1 chimeric promoter (1.039 kbp) and E9 polyadenylation sequence.  The partial suppression cassette in T-DNA I contains the sense segments of the FAD2-1A intron and FATB1-A 5' UTR, including the chloroplast targeting sequence, which are under the regulation of the seed 7Sα' promoter.

T-DNA II contains a partial suppression cassette, which consists of the antisense segment of FAD2-1A intron and FATB1-A 5′ UTR that is flanked by the H6 untranslated sequence.

During  plant transformation, a portion of the plants that were generated contained the two T-DNAs co-integrated at one locus in the soybean genome creating a DNA insert that contains a  cp4 epsps cassette and a single  FAD2-1A and FATB1-A suppression cassette.
LMO characteristics
  • Food
  • Feed
Additional Information