SPS-ØØF37-7 - Innate™ Ranger Russet Potato | BCH-LMO-SCBD-109055 | Living Modified Organism | Biosafety Clearing-House

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Living Modified Organism (LMO)
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Decisions on the LMO Risk Assessments  
last updated: 19 Oct 2015
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Innate™ Ranger Russet Potato
EN
F37
Yes
SPS-ØØF37-7
Simplot Innate™ Potato is genetically engineered to silence genes that lead to acrylamide formation in cooked potatoes, and genes that cause browning in damaged potatoes.
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
Var. Ranger Russet
EN
  • SPS-ØØF10-7 - Innate™ Ranger Russet Potato
    | J.R. Simplot Company | Changes in quality and/or metabolite content (Pigmentation / Coloration, Protein and amino acids)
Characteristics of the modification process
pSIM1278
EN
  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-106420-1 ADP glucose pyrophosphorylase gene promoter | Solanum tuberosum (Potato, SOLTU)
    Promoter
  • BCH-GENE-SCBD-106421-1 Asparagine synthetase-1 gene | Solanum tuberosum (Potato, SOLTU)
    Protein coding sequence | Changes in quality and/or metabolite content (Protein and amino acids)
  • BCH-GENE-SCBD-106424-3 Polyphenol oxidase 5 gene | Solanum verrucosum (SOLVR)
    Protein coding sequence | Changes in quality and/or metabolite content (Pigmentation / Coloration)
  • BCH-GENE-SCBD-14997-6 Granule bound starch synthase gene promoter | Solanum tuberosum (Potato, SOLTU)
    Promoter
  • BCH-GENE-SCBD-106426-1 Phosphorylase-L gene promoter | Solanum tuberosum (Potato, SOLTU)
    Promoter
  • BCH-GENE-SCBD-106425-1 Alpha-glucan water dikinase R1 gene promoter | Solanum tuberosum (Potato, SOLTU)
    Promoter
This DNA insert comprises two expression cassettes and was inserted into the pSIM1278 transformation vector.

The first cassette comprises fragments of both the asparagine synthetase-1 gene (Asn1) and the polyphenol oxidase-5 gene (Ppo5), arranged as inverted repeats between the Agp promoter of the ADP  glucose pyrophosphorylase gene (Agp) and the Gbss promoter of the granule-bound starch synthase gene (Gbss) and results in silencing of both the Ppo5 and Asn1 genes.

The second cassette is comprised of fragments of the promoters of the starch associated gene (R1) and the phosphorylase-L gene (PhL), operably linked to the same Agp and Gbss promoters as the first cassette. The function of the second cassette is to silence the promoters of the starch associated gene (R1) and the phosphorylase-L gene (PhL).

Southern blot analysis indicated that event F37 contains a single complete copy of the DNA insert and no vector backbone in the LMO.
EN
LMO characteristics
EN
  • Food
Detection method(s)
EN
Additional Information
EN
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