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Living Modified Organism
(LMO)
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Dicamba and Glufosinate Tolerant Maize
EN
MON87419
Yes
MON-87419-8
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Organization:Monsanto ()800 North Lindbergh Blvd.St. Louis, MO
63167, United States of AmericaPhone: + 1 314 694-1000,Fax: +1 314 694-3080,Email:Website: http://www.monsanto.com,
Maize modified with the insertion of transformation cassette containing the Dicamba monooxygenase (DMO) gene and Phosphinothricin N-acetyltransferase (PAT) gene to confer resistance to the herbicides Dicamba and Glufosinate respectively.
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-246-6 Organism Zea mays (Maize, Corn, MAIZE)Crops
EN
PV-ZMHT507801
EN
- Agrobacterium-mediated DNA transfer
1.640 kb
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0.100 kb
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1.040 kb
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0.550 kb
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0.210 kb
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0.432 kb
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0.060 kb
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0.479 kb
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0.215 kb
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1.022 kb
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0.209 kb
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-103899-3 Chloroplast Transit Peptide 4 | Petunia hybrida (Petunia, PETHY)Transit signal
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BCH-GENE-SCBD-100728-3 Dicamba monooxygenase gene | Stenotrophomonas maltophilia (S. maltophilia, Stenotrophomonas)Protein coding sequence | Resistance to herbicides
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BCH-GENE-SCBD-100356-6 Heat shock protein 17.3 terminator | Triticum aestivum (Wheat)Terminator
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BCH-GENE-SCBD-104662-3 PCSV Promoter | Peanut chlorotic streak virus (PCSV, PClSV)Promoter
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BCH-GENE-SCBD-100355-6 Rice actin 1, intron | Oryza sativa (Rice, ORYSA)Intron
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BCH-GENE-SCBD-111526-2 Ubiquitin gene promoter | Andropogon gerardii (Big bluestem, Turkeyfoot, Tall bluestem, Bluejoint, ANGER)Promoter
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BCH-GENE-SCBD-111528-2 Ubiquitin Intron Sequence | Andropogon gerardii (Big bluestem, Turkeyfoot, Tall bluestem, Bluejoint, ANGER)Intron
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BCH-GENE-SCBD-111527-2 Ubiquitin leader sequence | Andropogon gerardii (Big bluestem, Turkeyfoot, Tall bluestem, Bluejoint, ANGER)Leader Sequence
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BCH-GENE-SCBD-15002-4 Phosphinothricin N-acetyltransferase gene | Streptomyces viridochromogenes (STRVR)Protein coding sequence | Resistance to herbicides (Glufosinate)
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BCH-GENE-SCBD-111529-1 RA5B gene terminator | Oryza sativa (Rice, ORYSA)Terminator
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BCH-GENE-SCBD-100354-6 5' untranslated leader from chlorophyll a/b-binding protein | Triticum aestivum (Wheat)Leader sequence
The Streptomyces viridochromogenes phosphinothricin N-acetyltransferase (PAT) gene is under the control of the Andropogon gerardii ubiquitin (Ubq) gene promoter and the Oryza sativa alpha-amylase/trypsin inhibitor terminator. The transcript includes the A. gerardii 5' untranslated leader sequence and an intron from Ubq before the PAT.
The Stenotrophomonas maltophilia Dicamba monooxygenase (DMO) gene is under control of the peanut chlorotic streak caulimovirus (PC1SV) Full-length transcipt (FLt) promoter and the Triticum aestivum (wheat) heat shock protein 17 terminator. The transcript produced contains the wheat chlorophyll a/b-binding 5' untranslated leader sequence (for improved gene expression), the Oryza sativa Actin 1 untranslated region (UTR) and intron (for improved gene expression), the targeting and UTR of Petunia hybrida Chloroplast Transit Peptide 4, and DMO.
Note:
- Originally, the plasmid vector contained two T-DNA elements that were inserted during the initial transformation event: one containing the dmo and pat expression cassettes, and a second containing a cp4 epsps expression cassette. The cp4 epsps expression cassette is regulated by the Ract1 promoter from Oryza sativa, the Ract1 5′ untranslated leader from Oryza sativa, the Ract1 intron from Oryza sativa, the CTP2 targeting sequence from Arabidopsis thaliana, and the nos 3′ untranslated region from Agrobacterium tumefaciens. Subsequent traditional breeding, segregation, selection, and screening were used to isolate those plants that contain the dmo and pat expression cassettes (T-DNA I) and do not contain the cp4 epsps expression cassette (T-DNA II).
- Molecular characterization of MON 87419 indicated that a single copy of T-DNA I was integrated into the maize genome at a single intact locus that includes all expected elements within the insert, with the exception of incomplete Right and Left Border sequences. These analyses also showed no PV-ZMHT507801 backbone elements or T-DNA II sequences were present in the event.
EN
The Stenotrophomonas maltophilia Dicamba monooxygenase (DMO) gene is under control of the peanut chlorotic streak caulimovirus (PC1SV) Full-length transcipt (FLt) promoter and the Triticum aestivum (wheat) heat shock protein 17 terminator. The transcript produced contains the wheat chlorophyll a/b-binding 5' untranslated leader sequence (for improved gene expression), the Oryza sativa Actin 1 untranslated region (UTR) and intron (for improved gene expression), the targeting and UTR of Petunia hybrida Chloroplast Transit Peptide 4, and DMO.
Note:
- Originally, the plasmid vector contained two T-DNA elements that were inserted during the initial transformation event: one containing the dmo and pat expression cassettes, and a second containing a cp4 epsps expression cassette. The cp4 epsps expression cassette is regulated by the Ract1 promoter from Oryza sativa, the Ract1 5′ untranslated leader from Oryza sativa, the Ract1 intron from Oryza sativa, the CTP2 targeting sequence from Arabidopsis thaliana, and the nos 3′ untranslated region from Agrobacterium tumefaciens. Subsequent traditional breeding, segregation, selection, and screening were used to isolate those plants that contain the dmo and pat expression cassettes (T-DNA I) and do not contain the cp4 epsps expression cassette (T-DNA II).
- Molecular characterization of MON 87419 indicated that a single copy of T-DNA I was integrated into the maize genome at a single intact locus that includes all expected elements within the insert, with the exception of incomplete Right and Left Border sequences. These analyses also showed no PV-ZMHT507801 backbone elements or T-DNA II sequences were present in the event.
EN
- Food
- Feed
- MON-87419-8 - EU Reference Laboratory for GM Food and Feed (EURL-GMFF) ( JRC ) [ English ]
EN
EN
- MON87419 - ISAAA [ English ]
- MON87419 - APHIS [ English ]
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