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Living Modified Organism
(LMO)
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Roundup Ready™ maize
EN
NK603
Yes
MON-ØØ6Ø3-6
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Organization:Monsanto ()800 North Lindbergh Blvd.St. Louis, MO
63167, United States of AmericaPhone: + 1 314 694-1000,Fax: +1 314 694-3080,Email:Website: http://www.monsanto.com,
The maize (Zea mays) was modified for tolerance to glyphosate herbicides. To achieve glyphosate tolerance, the maize expresses enzyme 5-enolpyruvylshikimate-3-phosphate synthase, encoded by the cp4 epsps gene from Agrobacterium tumefaciens strain CP4, which has reduced binding affinity for glyphosate and allows continued synthesis of aromatic amino acids through the shikimate pathway in the presence of the herbicide.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-246-6 Organism Zea mays (Maize, Corn, MAIZE)Crops
EN
PV-ZMGT32
EN
- Biolistic / Particle gun
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0.800 kb
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0.600 kb
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0.200 kb
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1.400 kb
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0.300 kb
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0.600 kb
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0.800 kb
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0.200 kb
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1.400 kb
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0.300 kb
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-14979-7 5-enolpyruvylshikimate-3-phosphate synthase gene | Agrobacterium tumefaciens (Agrobacterium)Protein coding sequence | Resistance to herbicides (Glyphosate)
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BCH-GENE-SCBD-100364-5 Rice actin 1 gene promoter | Oryza sativa (Rice, ORYSA)Promoter
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BCH-GENE-SCBD-100355-6 Rice actin 1, intron | Oryza sativa (Rice, ORYSA)Intron
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BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminator
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BCH-GENE-SCBD-100359-7 Hsp70 intron | Zea mays (Maize, Corn, MAIZE)Intron
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BCH-GENE-SCBD-100365-6 Chloroplast transit peptide 2 | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)Transit signal
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BCH-GENE-SCBD-100366-6 CaMV Enhanced 35S promoter | Cauliflower mosaic virus (CaMV)Promoter
Information about the inserted DNA sequences
The DNA insert contains two adjacent genetic cassettes expressing the 5-enolpyruvylshikimate-3-phosphate synthase gene (CP4 EPSPS) from Agrobacterium tumefaciens strain CP4. Both cassettes were introduced by particle acceleration (biolistic) transformation.
(1) The cp4 epsps gene is under the regulation of the rice actin promoter (P-Ract1) and the rice actin intron (I-Ract1) and the 3' UTR region of the nopaline synthase gene (nos 3'). An intron from Oryza sativa (I-Ract1) was included to enhance expression of the cp4 epsps sequence from Agrobacterium tumefaciens, and Arabidopsis thaliana chloroplast transit peptide 2 to direct the translated CP4 EPSPS protein to the chloroplast.
(2) The cp4 epsps gene is regulated by the enhanced Cauliflower mosaic virus 35S promoter (CaMV P-e35S) and the 3' UTR region of the nopaline synthase gene (nos 3'). An intron from the Zea mays heat shock protein 70 (HSP70) was included to enhance expression of the cp4 epsps sequence from Agrobacterium tumefaciens, and Arabidopsis thaliana chloroplast transit peptide 2 to direct the translated CP4 EPSPS protein to the chloroplast.
Note
The DNA insert contains two adjacent genetic cassettes expressing the 5-enolpyruvylshikimate-3-phosphate synthase gene (CP4 EPSPS) from Agrobacterium tumefaciens strain CP4. Both cassettes were introduced by particle acceleration (biolistic) transformation.
(1) The cp4 epsps gene is under the regulation of the rice actin promoter (P-Ract1) and the rice actin intron (I-Ract1) and the 3' UTR region of the nopaline synthase gene (nos 3'). An intron from Oryza sativa (I-Ract1) was included to enhance expression of the cp4 epsps sequence from Agrobacterium tumefaciens, and Arabidopsis thaliana chloroplast transit peptide 2 to direct the translated CP4 EPSPS protein to the chloroplast.
(2) The cp4 epsps gene is regulated by the enhanced Cauliflower mosaic virus 35S promoter (CaMV P-e35S) and the 3' UTR region of the nopaline synthase gene (nos 3'). An intron from the Zea mays heat shock protein 70 (HSP70) was included to enhance expression of the cp4 epsps sequence from Agrobacterium tumefaciens, and Arabidopsis thaliana chloroplast transit peptide 2 to direct the translated CP4 EPSPS protein to the chloroplast.
Note
- The plasmid carrying the DNA transfer was digested by restriction enzyme MluI and only the fragments containing the two expression cassettes were inserted into the LM maize. The nptII gene encoding neomycin phosphotransferase II for kanamycin resistance and an origin of replication were not inserted.
- Maize line NK603 contains one insertion site containing a single copy of the linear DNA of PV-ZMGT32 used for transformation. Both cp4 epsps gene cassettes within the single insert which are intact.
- Promoter e35S is a 0.61Kb long sequence containing the promoter and leader for the CaMV 35S RNA containing the duplicated enhancer region.
EN
- Biofuel
- Feed
- Food
- MON-ØØ6Ø3-6 - EU Reference Laboratory for GM Food and Feed (EURL-GMFF) [ English ]
- MON-ØØ6Ø3-6 - CropLife International Detection Methods Database [ English ]
- MON-ØØ6Ø3-6 - EU Reference Laboratory for GM Food and Feed (EURL-GMFF) ( JRC ) [ English ]
- MON-ØØ6Ø3-6 - CropLife International Detection Methods Database ( CropLife ) [ English ]
EN
Glyphosate specifically binds to and inactivates the enzyme EPSPS, which is part of an important plant biochemical pathway called the shikimate pathway. The shikimate pathway is involved in the biosynthesis of the aromatic amino acids tyrosine, phenylalanine and tryptophan, as well as other aromatic compounds. When conventional plants are treated with glyphosate they cannot produce the aromatic amino acids essential to their survival. The modified maize line permits farmers to use glyphosate-containing herbicides for weed control in the cultivation of maize. The EPSPS enzyme is present in all plants, bacteria and fungi, but not in animals, which do not synthesize their own aromatic amino acids. Thus, EPSPS is normally present in food derived from plant and microbial sources.
- EUginius: NK603 [URL] [ English ]
- OECD Biotrack database: NK603 [URL] [ English ]
- BATS (2003). Genetically Modified (GM) Crops: Molecular and Regulatory details, version 2 [PDF] [ English ]
- ANZFA (2002). A416 Final Assessment Report - Glyophaste-tolerant Corn Line NK603 [PDF] ( FSANZ, Food Standards Australia New Zealand, Australia New Zealand Food Authority ) [ English ]
- EFSA (2025). Assessment of maize NK603 [PDF] ( renewal authorisation, Regulation (EC) No 1829/2003 (dossier GMFF-2023-21250) ) [ English ]
- EU Commission FFID. Field trials for identification, characterization and agronomical evaluation for registration purposes in the list of O.E.V.V. vegetal corn varieties derived form NK603 line, tolerant to to glyphosate herbicide [URL] [ English ]
- Patent (2007). Corn event PV-ZMGT32(nk603) and compositions and methods for detection thereof [PDF] ( US20070292854A1 ) [ English ]
| Record type | Field | Record(s) | |
|---|---|---|---|
| Living Modified Organism | Recipient Organism or Parental Organisms | 59 | |
| Living Modified Organism | Related LMO(s) | 2 | |
| Country's Decision or any other Communication | LMO identification | 101 | |
| Risk Assessment generated by a regulatory process | Living modified organism(s) | 113 | |
| Laboratory for detection and identification of LMOs | LMO(s) detectable by the laboratory | 34 | |
