NMK-89185-6 - New Leaf™ Plus Russet Burbank potato | BCH-LMO-SCBD-14897 | Living Modified Organism | Biosafety Clearing-House


Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
last updated: 25 Apr 2013
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
New Leaf™ Plus Russet Burbank potato
Potatoes with insect-resistance and resistance to potato leafroll virus through inclusion of the cry3A gene from Bacillus thuringiensis which confers resistance to coleopteran pests, and DNA sequences corresponding to potato leafroll virus (PLRV) helicase and replicase domains which confer resistance to PLRV.  The npt II gene confers tolerance to the antibiotic kanamycin is used as a selection marker. 
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
  • NMK-89684-1 - New Leaf™ Plus Russet Burbank potato
    | Monsanto | Resistance to antibiotics (Kanamycin), Resistance to diseases and pests (Insects, Coleoptera (beetles), Viruses, Potato leaf roll virus (PLRV))
  • New Leaf™ Plus Russet Burbank potato
    | Monsanto | Resistance to antibiotics (Kanamycin), Resistance to diseases and pests (Insects, Coleoptera (beetles), Viruses, Potato leaf roll virus (PLRV))
Characteristics of the modification process
  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-14989-5 Cry3A | Bacillus thuringiensis (Bt, Bacillus, BACTU)
    Protein coding sequence | Resistance to diseases and pests (Insects, Coleoptera (beetles))
  • BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)
    Protein coding sequence | Resistance to antibiotics (Kanamycin)
  • BCH-GENE-SCBD-103851-5 rbcS Promoter | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)
  • BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)
  • BCH-GENE-SCBD-101507-5 FMV 34S promoter | Figwort mosaic virus (Figwort mottle virus, FMV, CMoVb)
  • BCH-GENE-SCBD-103922-2 HSP17.9 Leader Sequence | Glycine max (Soybean, Soya bean, Soya, SOYBN)
  • BCH-GENE-SCBD-104847-2 PLRV Replicase gene | Potato leafroll virus (PLRV)
    Protein coding sequence | Resistance to diseases and pests (Viruses, Potato leaf roll virus (PLRV))
  • BCH-GENE-SCBD-101877-5 rbcS-E9 gene terminator | Pisum sativum (Garden pea, PEA)
  • BCH-GENE-SCBD-100270-6 Nopaline Synthase Gene Promoter | Agrobacterium tumefaciens (Agrobacterium)
The coding sequence of the Cry3A gene was modified to plant preferred codons. This resulted in changes to 399 of 1791 nucleotides but there were no changes to the resulting amino acid sequence.

PCR analysis indicated that the transformation cassette was inserted into the host genome in the same orientation as in the vector. Additionally no fragments outside the left and right border were detected.
LMO characteristics
  • Food
Detection method(s)
Additional Information
Resistance to attack by CPB was accomplished by introducing the cry3A gene from Bacillus thuringiensis subsp. tenebrionis, which encodes an insecticidal crystalline Cry3A delta-endotoxin protein. The insecticidal activity of Cry3A protein is due to its selective binding to specific sites localized on the brush border midgut epithelium of susceptible insect species. Following binding, cation-specific pores are formed that disrupt midgut ion flow and thereby cause gut paralysis, ultimately leading to bacterial sepsis and death. Delta-endotoxins, such as the Cry3A protein expressed in CPB resistant potato lines, exhibit highly selective insecticidal activity against a narrow range of coleopteran insects such as CPB, elm leaf beetle and yellow mealworm. Their specificity of action is directly attributable to the presence of specific receptors in the target insects.

Introducing DNA sequences corresponding to the ORF-1 and ORF-2 regions from PLRV conferred resistance to PLRV infection. These two ORFs encode the putative viral helicase and replicase domains that are required for viral RNA synthesis. The introduced viral sequences do not result in the formation of any infectious particles, nor does their expression result in any disease pathology. These transgenic potato cultivars exhibit the trait of resistance to infection and subsequent disease caused by PLRV through an incompletely understood process that has been termed "replicase-mediated resistance", which may involve silencing of viral gene translation.