MON-88913-8 x MON-15985-7 - Roundup Ready™ Flex™ Bollgard II™ cotton | BCH-LMO-SCBD-15215 | Living Modified Organism | Biosafety Clearing-House

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Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
last updated: 12 Feb 2014
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Roundup Ready™ Flex™ Bollgard II™ cotton
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88913 x 15985 (MON88913 x MON15985)
MON-88913-8 x MON-15985-7
  • - Organization: Monsanto () | BCH-CON-SCBD-14925-3
    Organization
    Monsanto ()
    800 North Lindbergh Blvd.
    St. Louis, MO
    63167, United States of America
    Phone: + 1 314 694-1000,
    Fax: +1 314 694-3080,
    Email:
The stacked cotton line MON88913 x MON15985 expresses several novel proteins: the delta-endotoxins the Cry1Ac and Cry2Ab which confer resistance to lepidopteran pests, and EPSPS (5-enolpyruvylshikimaete-3-phosphate synthase) that confers tolerance to the herbicide glyphosate.

The cry1Ac gene and the cry2Ab gene from Bacillus thuringiensis subsp. kurstaki conferring resistance to lepidopteran pests, and the epsps gene encoding 5-enolpyruvylshikimaete-3-phosphate synthase that confers tolerance to the herbicide glyphosate. gene from Agrobacterium tumefaciens strain CP4.

The cry1Ac and cry2Ab genes, isolated from the common soil bacterium Bacillus thuringiensis (Bt) strain subsp. kurstaki, produce the insect control proteins (delta-endotoxins) Cry1Ac and Cry2Ab. Cry proteins, of which these are only two among many, act by selectively binding to specific sites localized on the lining of the midgut of susceptible insect species. Following binding, pores are formed that disrupt midgut ion flow, causing gut paralysis and eventual death due to bacterial sepsis. There are no binding sites for the delta-endotoxins of B. thuringiensis on the surface of mammalian intestinal cells, therefore, livestock animals and humans are not susceptible to these proteins.

The line also contains a form of the plant enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) that allows the plant to survive the otherwise lethal application of glyphosate. The glyphosate-tolerant EPSPS gene was isolated from the CP4 strain of the common soil bacterium Agrobacterium tumefaciens.

Glyphosate specifically binds to and inactivates the enzyme EPSPS, which is part of an important plant biochemical pathway called the shikimate pathway. The shikimate pathway is involved in the biosynthesis of the aromatic amino acids tyrosine, phenylalanine and tryptophan, as well as other aromatic compounds. When conventional plants are treated with glyphosate they cannot produce the aromatic amino acids essential to their survival. The modified maize line permits farmers to use glyphosate-containing herbicides for weed control in the cultivation of maize. The EPSPS enzyme is present in all plants, bacteria and fungi, but not in animals, which do not synthesize their own aromatic amino acids. Thus, EPSPS is normally present in food derived from plant and microbial sources.
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The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
  • BCH-ORGA-SCBD-12080-6 Organism Gossypium hirsutum (Cotton)
    Crops
  • BCH-LMO-SCBD-15168-16 Living Modified Organism MON-88913-8 - Roundup Ready™ Flex™ cotton
    Resistance to herbicides - Glyphosate
  • BCH-LMO-SCBD-14774-18 Living Modified Organism MON-15985-7 - Bollgard II™ cotton
    Resistance to antibiotics - Kanamycin, Streptomycin Resistance to diseases and pests - Insects - Lepidoptera (butterflies and moths) Selectable marker genes and reporter genes
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Characteristics of the modification process
PV-GHGT35, PV-GHBK04 and PV-GHBK11
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  • Cross breeding
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
A stacked insect-resistant and herbicide-tolerant cotton derived by crossing MON-88913-8 with MON-15985-7, including the cry1Ac gene and the cry2Ab gene from Bacillus thuringiensis subsp. kurstaki conferring resistance to lepidopteran pests, and the epsps gene encoding 5-enolpyruvylshikimaete-3-phosphate synthase that confers tolerance to the herbicide glyphosate. The neomycin phosphotransferase II (npt II) gene confers resistance to the antibiotic kanamycin and was used as a selectable marker.

DNA insert from MON 88913 vector PV-GHGT35
Roundup Ready® Flex cotton (MON 88913) was developed to allow the use of glyphosate, the active ingredient in the herbicide Roundup®, as a weed control option in cotton production. This genetically engineered cotton contains a novel form of the plant enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) that allows MON 88913 to survive otherwise lethal applications of glyphosate. The EPSPS gene introduced into MON 88913 was isolated from a strain of the common soil bacterium Agrobacterium tumefaciens strain CP4; the EPSPS enzyme expressed by this gene is tolerant to glyphosate. MON 88913 cotton contains two copies of the EPSPS gene to confer tolerance to glyphosate later in the growing season, specifically after the fifth true leaf stage. 

DNA insert from MON15985 vector PV-GHBK11 and PV-GHBK04
Event 15985 (tradename Bollgard II®) was derived from the retransformation of transgenic cotton line MON 531. As a result of these two transformation events, MON15985 contains the cry1Ac gene and the cry2Ab conferring resistance to lepidopteran pests as well as copies of the nptII and uidA and aadA genes.

For additional information on this LMO, please refer to the records of the parental LMOs.
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LMO characteristics
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  • Food
  • Feed
  • Fiber/textile
Additional Information
Records referencing this document Show in search
Record type Field Record(s)
Risk Assessment generated by a regulatory process Living modified organism(s) 92
Country's Decision or any other Communication Living modified organism(s) 90
Laboratory for detection and identification of LMOs LMO(s) detectable by the laboratory 2
Risk Assessment generated by an independent or non-regulatory process Living modified organism(s) 1
Living Modified Organism Related LMO(s) 1