Canola MPS964 Phytaseed™ (phytase-producing) | BCH-LMO-SCBD-15382 | Living Modified Organism | Biosafety Clearing-House


Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
last updated: 18 Sep 2012
Living Modified Organism identity
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Canola MPS964 Phytaseed™ (phytase-producing)
Canola lines MPS961, MPS962, MPS963, MPS964 and MPS965 have been modified to allow the plant to produce a fungal 3-phytase. This enzyme can be utilized to increase the breakdown of plant phytates which bind phosphorus. Phytate is the major storage form of phosphorus in many seeds and phytate-bound phosphorus is unavailable to monogastric animals. Since monogastric animals are not able to degrade this molecule, much of the phosphorus bound to phytate passes into the environment through the manure. Use of the enzyme and appropriate management techniques can lead to a reduction in the phosphorus content of manure, thus improving environmental conditions.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
  • Canola MPS961 Phytaseed™ (phytase-producing )
    | Changes in quality and/or metabolite content, Resistance to antibiotics (Kanamycin)
  • Canola MPS962 Phytaseed™ (phytase-producing )
    | Changes in quality and/or metabolite content, Phytate degradation, Resistance to antibiotics (Kanamycin)
  • Canola MPS963 Phytaseed™ (phytase-producing)
    | Changes in quality and/or metabolite content, Phytate degradation
  • Canola MPS965 Phytaseed™ (phytase-producing)
    | Changes in quality and/or metabolite content, Phytate degradation, Resistance to antibiotics (Kanamycin)
Characteristics of the modification process
  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-15378-5 phyA gene | Aspergillus niger
    Protein coding sequence | Changes in quality and/or metabolite content,Phytate degradation
  • BCH-GENE-SCBD-103918-3 Cruciferin A gene promoter | Brassica napus (Turnip, Rapeseed, Canola Plant, Oilseed Rape, Rape, BRANA)
  • BCH-GENE-SCBD-103919-1 Cruciferin A gene terminator | Brassica napus (Turnip, Rapeseed, Canola Plant, Oilseed Rape, Rape, BRANA)
  • BCH-GENE-SCBD-100270-6 Nopaline Synthase Gene Promoter | Agrobacterium tumefaciens (Agrobacterium)
  • BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)
    Protein coding sequence | Resistance to antibiotics (Kanamycin)
  • BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)
The phytase gene was obtained from Aspergillus niger var. van Tieghem, and expression of this gene produces the enzyme 3-phytase. The nptII gene was isolated from transposon Tn5 in Klebsiella pneumonia. The nptII protein, neomycin phosphotransferase II, confers resistance to some aminoglycoside antibiotics including neomycin and kanamycin, and was used by the firm as a selectable marker for transformed plant cells.

The novel genetic material in the new canola lines was inserted into the canola variety, Westar, using Agrobacterium tumefaciens-mediated transformation with the disarmed Ti-plasmid pMOG625. The T-DNA contained both phytase and nptII genes. The phytase gene is under the control of the cruciferin A seed storage protein transcript promoter which includes a cruciferin signal peptide sequence. Its terminator is also from the cruciferin A seed storage protein transcript. Both controlling sequences were obtained from Brassica napus. The nptII gene is under the control of the NOS promoter and terminator with an Agrobacterium tumefaciens-derived open reading frame inserted between the gene and its terminator. The open reading frame consists of coding for 50 amino acids from the Agrobacterium ornithine-cyclo-deaminase.
LMO characteristics
  • Food
  • Feed
Detection method(s)
Lines MPS961, MPS962, MPS963 and MPS964 do not carry the bacterial selection marker nptII, nor the bacterial origin of replication as demonstrated by Southern analyses. Only MPS965 of the transgenic lines contains an expressed neomycin phosphotransferase II gene.
Additional Information