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Living Modified Organism
(LMO)
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
VECTORMUNE® FP-MG + AE
EN
Vectormune FP-MG + AE
No
VECTORMUNE® FP-MG+AE
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Organization:Ceva Saúde Animal Ltda (CEVA)Private sector (business and industry)Rua Manuel Joaquim Filho, 303 Pulínia - SPPaulínia, São Paulo
13140-000, BrazilPhone: 551938337700,Fax: 55193833-7722,Email: alexisgoux@ceva.com,Website: http://www.ceva.com.br/,
VECTORMUNE® FP MG + AE is a genetically modified live virus vaccine for the vaccination of chickens as an aid in the prevention of fowl pox and Mycoplasma gallisepticum. The fowl pox vaccine has been genetically engineered to contain and express key protective Mycoplasma gallisepticum bacterial antigens, the MG 40k and the mgc3 genes. In addition to the fowlpox virus vector, this freeze-dried vaccine contains a conventional live avian encephalomyelitis (AE) virus for use as an aid in the prevention of avian encephalomyelitis.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-105337-2 Organism Mycoplasma gallisepticum (MG, MYCGL)Bacteria
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BCH-LMO-SCBD-105419-1 Living Modified Organism VECTORMUNE® FP MG VaccineCeva Saúde Animal Ltda(CEVA) | Production of medical or pharmaceutical compounds (human or animal) (Vaccines)
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BCH-ORGA-SCBD-105171-4 Organism Fowlpox virus (FOWPV)Viruses
Fowlpox virus strain Cutter
EN
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VECTORMUNE® FP MG Vaccine| Ceva Saúde Animal Ltda(CEVA) | Production of medical or pharmaceutical compounds (human or animal) (Vaccines)
pUC18
EN
- Virus-mediated gene transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-105411-1 MG 40k antigen gene | Mycoplasma gallisepticum (MG, MYCGL)Protein coding sequence | Production of medical or pharmaceutical compounds (human or animal) (Vaccines)
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BCH-GENE-SCBD-105360-1 mgc3 gene | Mycoplasma gallisepticum (MG, MYCGL)Protein coding sequence | Production of medical or pharmaceutical compounds (human or animal) (Vaccines)
Information on the DNA sequences
The DNA insert to the vector comprised two genetic cassettes expressing the MG 40K and mcg3 genes derived from Mycoplasma gallisepticum.
(1) The expression of the MG 40K gene is directed by the synthetic Ps promoter, which emulates the consensus early/late promoter of poxvirus, and a termination sequence derived from the gB gene of the Marek Disease Virus (MDV) was also inserted into the pUC18 vector. A MDV gB transit signal sequence was added to the 3' terminus of the of MG 40K and mcg3 coding sequences to ensure localization to the cell membrane.
(2) The expression of the mcg3 gene was similarly under the control of the synthetic Ps promoter, the MDV gB transit signal sequence, and the gB terminator.
Note
The DNA insert to the vector comprised two genetic cassettes expressing the MG 40K and mcg3 genes derived from Mycoplasma gallisepticum.
(1) The expression of the MG 40K gene is directed by the synthetic Ps promoter, which emulates the consensus early/late promoter of poxvirus, and a termination sequence derived from the gB gene of the Marek Disease Virus (MDV) was also inserted into the pUC18 vector. A MDV gB transit signal sequence was added to the 3' terminus of the of MG 40K and mcg3 coding sequences to ensure localization to the cell membrane.
(2) The expression of the mcg3 gene was similarly under the control of the synthetic Ps promoter, the MDV gB transit signal sequence, and the gB terminator.
Note
- A 3kb Hpal-Spel fragment of the Fowl Pox Virus (FPV) genome was inserted into a EcoR1-HindIII site of a pUC18 vector. The homologous recombination site may be interrupting a possible open reading frame but is thought to be non-essential for viral replication and has no known function in the FPV.
- A 175bp EcoRV fragment of the FPV genome was removed from this construct and replaced with the MG 40K and mcg3 coding sequences.
- Mycoplasma gallisepticum strains S6 and R are mentioned as donors, but the specific strain of the vaccine's the MG 40K and the mgc3 sequences are derived from are not identified.
- This construct and the attenuated parental FPV strain were co-transformed into chicken embryo fibroblasts (CEF) and incubated under conditions that favor homologous recombination.
- After transfection, viral particles were cultivated from the chicken embryo fibroblasts and assayed for expression of MG 40K and mcg3 proteins. Plaques expressing MG 40K and mcg3 proteins were isolated and selected until the pure recombined virus was obtained.
EN
- Vaccine
EN
The vaccine contains a genetically modified live virus (prevention of Boubabaum for FowlpoxV virus) and Mycoplasma galliseptic genes by inserting this bacterium into the virus. The BA virus was modified by genetic engineering and expressed key protective MG antigens. The vaccine is presented in lyophilized form and is recommended for active immunization of healthy chickens to protect against Fowl Pox and MG, for administration via puncture in the wing membrane. A conventional attenuated sample of avian encephalomyelitis (AE) will be added to this vaccine.
- CTNBio Technical Opinion 2226/2009: Developer's Request on biosafety of a genetically modified organism for activities of Vectormune FP MG AE [PDF] ( Ministério da Ciência, Tecnologia, Inovações e Comunicações (MCTI); Solicitação de Aprovação de Liberação Comercial; Comissão técnica nacional de biossegurança; National Technical Commission on Biosafety; Parecer Técnico Nº 2226/2009 ) [ English ]
- USDA-APHIS (2019): Summary of Studies Supporting USDA Product Licensure – Avian Encephalomyelitis-Fowl Pox-Mycoplasma Gallisepticum Vaccine, Live Virus, Live Fowl Pox Vector [PDF] ( Product Compilation Summary 368-1D61R0 ) [ English ]
- CEVA leaflet: Vectormune FP MG AE [PDF] [ English ]
