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Living Modified Organism
(LMO)
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Spring barley with altered stomatal function and ozone sensitivity (hv_ost1)
EN
hv_ost1
No
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Klopina,Organization:Usovsko ()Private sector (business and industry)
78973, CzechiaPhone: +420 583 484 111,Fax:Email: klopina@usovsko.cz,Website: http://usovsko.cz/?p=o-nas,
The spring barley (Hordeum vulgare) was modified for altered stomatal function and sensitivity to ozone. OST1 encodes a SnRK2-type protein kinase involved in abscisic acid-mediated guard-cell signaling and stomatal closure. Modification of this locus alters stomatal conductance and may affect ozone uptake and ozone sensitivity, since ozone entry into leaves is strongly influenced by stomatal aperture and conductance. The line is intended for research on the relationship between stomatal regulation, ozone response and barley performance.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-12110-5 Organism Hordeum vulgare (Barley, HORVU)Crops
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Sm13-cas9-ec64420_Barley
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- Gene editing (e.g. CRISPR-Cas, etc.)
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-299563-2 OST1 | Hordeum vulgare (Barley, HORVU)Protein coding sequence | Changes in physiology and/or production (Growth rate)
Information on the modified DNA sequence
The hv_ost1 line was developed through CRISPR/Cas-mediated targeted mutagenesis of HORVU.MOREX.r3.4HG0342860, an approximately 2-kb gene located on barley chromosome 4H and comprising eight exons. The gene encodes an OST1-related member of the SNF1-related protein kinase 2 (SnRK2) family. OST1-like kinases participate in guard-cell signalling and the regulation of stomatal closure in response to abscisic acid and environmental conditions.
CRISPR/Cas-mediated mutagenesis produced a 55-bp deletion in the first exon, resulting in functional inactivation of the target gene. This modification is associated with increased stomatal conductance and consequently increased ozone uptake, as well as reduced stomatal responsiveness to abscisic acid and changes in vapour-pressure deficit. The progeny selected for the field trial does not contain transgenic sequences originating from the expression vector used to induce the targeted mutation.
The hv_ost1 line was developed through CRISPR/Cas-mediated targeted mutagenesis of HORVU.MOREX.r3.4HG0342860, an approximately 2-kb gene located on barley chromosome 4H and comprising eight exons. The gene encodes an OST1-related member of the SNF1-related protein kinase 2 (SnRK2) family. OST1-like kinases participate in guard-cell signalling and the regulation of stomatal closure in response to abscisic acid and environmental conditions.
CRISPR/Cas-mediated mutagenesis produced a 55-bp deletion in the first exon, resulting in functional inactivation of the target gene. This modification is associated with increased stomatal conductance and consequently increased ozone uptake, as well as reduced stomatal responsiveness to abscisic acid and changes in vapour-pressure deficit. The progeny selected for the field trial does not contain transgenic sequences originating from the expression vector used to induce the targeted mutation.
EN
- Research
The targeted mutation can be detected by PCR amplification of the modified genomic region and confirmed by sequence analysis, enabling identification of the specific edited allele.
EN
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