Digestibility test showed that DMO protein, PAT protein, and CP4 ESPS protein are readily degraded in either pepsin or pancreatic enzyme unlikely to pose a human and animal health concern. In the case of FT_T protein, it was readily degraded in pancreatic enzyme but not in pepsin enzyme alone. However, during the sequential digestion, pepsin enzymes became easily degraded. The assessors further noted that DMO, PAT,  and FT_T are functionally inactivated and degraded by heat treatment at 55°C and above at 15 min or beyond, while the same analyses demonstrated the inactivation of the CP4 EPSPS protein at temperatures above 75°C for exposures at 15 minutes. Additionally, the results of acute oral gavage showed that there were no test substance-related differences in mean body weights, mean body weight changes, or mean food consumption when MON 87429 DMO protein was administered by oral gavage at a dose of 1000 mg/kg body weight in male and female CD-1 mice. This is the same for PAT protein and FT_T protein that were both administered at a dose of 2000 mg/kg body weight in male and female CD-1 mice. There were also no treatment related adverse effects observed in animals dosed with CP4 EPSPS protein at 572 mg/kg body weight. The novel proteins were also compared to allergen and toxin databases and no biologically significant similarities were noted. 

In consideration of the unlikely and the results of the toxicological and allergenicity assessments of the novel proteins DMO, PAT, FT_T, and CP4 EPSPS, the assessors deemed that the overall risk for Corn MON87429 is negligible.