IND-ØØ412-7 - Drought-tolerant wheat (HB4 wheat) | BCH-LMO-SCBD-259261 | Living Modified Organism | Biosafety Clearing-House

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Living Modified Organism (LMO)
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Decisions on the LMO Risk Assessments  
last updated: 18 Feb 2022
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Drought-tolerant wheat (HB4 wheat)
EN
IND00412
Yes
IND-ØØ412-7
The wheat (Triticum aestivum) was modified for abiotic stress tolerance and herbicide resistance. For tolerance to drought and salinity, the wheat expresses Helianthus annuus homeodomain-leucine zipper 4, which enhances the plant's natural abiotic stress response. For tolerance to glufosinate, the wheat expresses Streptomyces hygroscopicus phosphinothricin N-acetyltransferase, which inactivates glufosinate herbicides through acetylation.
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
EN
Characteristics of the modification process
pIND-HB4 and pIND-Bar
EN
  • Biolistic / Particle gun
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
The wheat contains two gene cassettes: Helianthus annuus homeodomain-leucine zipper 4 (hb4) from pIND-HB4 and Streptomyces hygroscopicus phosphinothricin N-acetyltransferase (bar) from pIND-Bar.

The hb4 coding sequence is under control of a Zea mays ubiquitin promoter and Agrobacterium tumefaciens nopaline synthase terminator. Due to the constitutive nature of the promoter, high levels of expression are expected in all plant tissues. The ubiquitin promoter additionally contains the first exon and intron of the ubiquitin coding sequence, which further enhance expression of the hb4 coding sequence.

Similarly, the bar coding sequence is under control of a Zea mays ubiquitin promoter and Agrobacterium tumefaciens nopaline synthase terminator. Due to the constitutive nature of the promoter, high levels of expression are expected in all plant tissues. The ubiquitin promoter additionally contains the first exon and intron of the ubiquitin coding sequence, which further enhance expression of the bar coding sequence. 
EN
LMO characteristics
EN
Detection method(s)
EN
Additional Information
The wheat is also known under as IND412, HB4 and 412.
EN
Records referencing this document Show in search
Record type Field Record(s)
Risk Assessment generated by a regulatory process Living modified organism(s) 3
Country's Decision or any other Communication Living modified organism(s) 2