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Living Modified Organism (LMO)
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Drought-tolerant wheat (HB4 wheat)
EN
IND00412
Yes
IND-ØØ412-7
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Rosario,Organization:INDEAR S.A. ()
, ArgentinaPhone: (+54) 0341-486 1100,Fax:Email: info@indear.com,Website:
The wheat (Triticum aestivum) was modified for abiotic stress tolerance and herbicide resistance. For tolerance to drought and salinity, the wheat expresses Helianthus annuus homeodomain-leucine zipper 4, which enhances the plant's natural abiotic stress response. For tolerance to glufosinate, the wheat expresses Streptomyces hygroscopicus phosphinothricin N-acetyltransferase, which inactivates glufosinate herbicides through acetylation.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-45396-4 Organism Triticum aestivum (Wheat)Crops
EN
pIND-HB4 and pIND-Bar
EN
- Biolistic / Particle gun
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-100362-7 Ubiquitin gene promoter | Zea mays (Maize, Corn, MAIZE)Promoter
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BCH-GENE-SCBD-103627-5 Ubiquitin Intron 1 | Zea mays (Maize, Corn, MAIZE)Intron
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BCH-GENE-SCBD-112017-1 Homeodomain-leucine zipper 4 gene | Helianthus annuus (Sunflower, HELAN)Protein coding sequence | Tolerance to abiotic stress (Drought, Salinity)
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BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminator
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BCH-GENE-SCBD-14972-12 Phosphinothricin N-acetyltransferase gene | Streptomyces hygroscopicus (STRHY)Protein coding sequence | Resistance to herbicides (Glufosinate)
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BCH-GENE-SCBD-259256-1 Ubiquitin exon 1 | Zea mays (Maize, Corn, MAIZE)Protein coding sequence | Enhanced gene expression
The wheat contains two gene cassettes: Helianthus annuus homeodomain-leucine zipper 4 (hb4) from pIND-HB4 and Streptomyces hygroscopicus phosphinothricin N-acetyltransferase (bar) from pIND-Bar.
The hb4 coding sequence is under control of a Zea mays ubiquitin promoter and Agrobacterium tumefaciens nopaline synthase terminator. Due to the constitutive nature of the promoter, high levels of expression are expected in all plant tissues. The ubiquitin promoter additionally contains the first exon and intron of the ubiquitin coding sequence, which further enhance expression of the hb4 coding sequence.
Similarly, the bar coding sequence is under control of a Zea mays ubiquitin promoter and Agrobacterium tumefaciens nopaline synthase terminator. Due to the constitutive nature of the promoter, high levels of expression are expected in all plant tissues. The ubiquitin promoter additionally contains the first exon and intron of the ubiquitin coding sequence, which further enhance expression of the bar coding sequence.
The hb4 coding sequence is under control of a Zea mays ubiquitin promoter and Agrobacterium tumefaciens nopaline synthase terminator. Due to the constitutive nature of the promoter, high levels of expression are expected in all plant tissues. The ubiquitin promoter additionally contains the first exon and intron of the ubiquitin coding sequence, which further enhance expression of the hb4 coding sequence.
Similarly, the bar coding sequence is under control of a Zea mays ubiquitin promoter and Agrobacterium tumefaciens nopaline synthase terminator. Due to the constitutive nature of the promoter, high levels of expression are expected in all plant tissues. The ubiquitin promoter additionally contains the first exon and intron of the ubiquitin coding sequence, which further enhance expression of the bar coding sequence.
EN
EN
The wheat is also known under as IND412, HB4 and 412.
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