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Organismos vivos modificados
(LMO)
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Spring barley with altered stomatal function and ozone sensitivity (hv_ost1)
EN
hv_ost1
No
-
Klopina,Organización:Usovsko ()Sector privado (comercio e industria)
78973, ChequiaTeléfono: +420 583 484 111,Fax:Correo electrónico: klopina@usovsko.cz,Sitio web: http://usovsko.cz/?p=o-nas,
The spring barley (Hordeum vulgare) was modified for altered stomatal function and sensitivity to ozone. OST1 encodes a SnRK2-type protein kinase involved in abscisic acid-mediated guard-cell signaling and stomatal closure. Modification of this locus alters stomatal conductance and may affect ozone uptake and ozone sensitivity, since ozone entry into leaves is strongly influenced by stomatal aperture and conductance. The line is intended for research on the relationship between stomatal regulation, ozone response and barley performance.
El término "organismo receptor" hace referencia a un organismo (modificado o no modificado) que fue objeto de una modificación genética, en tanto que "organismos parentales" hace referencia a los implicados en el cruzamiento o la fusión de células.
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BCH-ORGA-SCBD-12110-5 Organismo Hordeum vulgare (Barley, HORVU)Cultivos
ES
Sm13-cas9-ec64420_Barley
EN
- Edición génica (por ej, CRISPR-Cas, etc.)
Algunos de estos elementos genéticos pueden estar presentes como fragmentos o formas truncadas. Por favor, véanse las notas a continuación, en los casos en los que corresponda.
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BCH-GENE-SCBD-299563-2 OST1 | Hordeum vulgare (Barley, HORVU)Secuencia de codificación de proteínas | Cambios en la fisiología y/o producción (Índice de crecimiento)
Information on the modified DNA sequence
The hv_ost1 line was developed through CRISPR/Cas-mediated targeted mutagenesis of HORVU.MOREX.r3.4HG0342860, an approximately 2-kb gene located on barley chromosome 4H and comprising eight exons. The gene encodes an OST1-related member of the SNF1-related protein kinase 2 (SnRK2) family. OST1-like kinases participate in guard-cell signalling and the regulation of stomatal closure in response to abscisic acid and environmental conditions.
CRISPR/Cas-mediated mutagenesis produced a 55-bp deletion in the first exon, resulting in functional inactivation of the target gene. This modification is associated with increased stomatal conductance and consequently increased ozone uptake, as well as reduced stomatal responsiveness to abscisic acid and changes in vapour-pressure deficit. The progeny selected for the field trial does not contain transgenic sequences originating from the expression vector used to induce the targeted mutation.
The hv_ost1 line was developed through CRISPR/Cas-mediated targeted mutagenesis of HORVU.MOREX.r3.4HG0342860, an approximately 2-kb gene located on barley chromosome 4H and comprising eight exons. The gene encodes an OST1-related member of the SNF1-related protein kinase 2 (SnRK2) family. OST1-like kinases participate in guard-cell signalling and the regulation of stomatal closure in response to abscisic acid and environmental conditions.
CRISPR/Cas-mediated mutagenesis produced a 55-bp deletion in the first exon, resulting in functional inactivation of the target gene. This modification is associated with increased stomatal conductance and consequently increased ozone uptake, as well as reduced stomatal responsiveness to abscisic acid and changes in vapour-pressure deficit. The progeny selected for the field trial does not contain transgenic sequences originating from the expression vector used to induce the targeted mutation.
ES
- Investigación
The targeted mutation can be detected by PCR amplification of the modified genomic region and confirmed by sequence analysis, enabling identification of the specific edited allele.
ES
| Tipo de registro | Campo | Registro(s) | |
|---|---|---|---|
| Organismos vivos modificados | OVM relacionado(s) | 1 | |
| Evaluación del riesgo generada por un proceso regulatorio | Organismo(s) vivo(s) modificado(s) | 1 | |
