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Organismos vivos modificados
(LMO)
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Spring barley with a mutation in the VRN1 gene (vrn1_LOF_76del)
EN
vrn1_LOF_76del
No
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Prague,Organización:Institute of Experimental Botany, AS CR (IEB)Instituto académico o de investigación
, ChequiaTeléfono: + 420 225 106 455,Fax:Correo electrónico: ueb@ueb.cas.cz,Sitio web: http://www.ueb.cas.cz/en,
The spring barley (Hordeum vulgare L.) was modified by CRISPR/Cas-mediated targeted mutagenesis of the endogenous HvVRN1 (vernalization1) gene to alter flowering and vernalization responses. The vrn1_LOF_76del line contains a targeted 76 bp deletion in the first exon of HvVRN1. This deletion disrupts the reading frame, preventing production of a functional HvVRN1 protein and resulting in a loss-of-function allele. HvVRN1 encodes an APETALA1/FRUITFULL-like MADS-box transcription factor that functions as a key regulator of the transition from vegetative to reproductive growth and integrates vernalization signals to promote flowering in barley.
El término "organismo receptor" hace referencia a un organismo (modificado o no modificado) que fue objeto de una modificación genética, en tanto que "organismos parentales" hace referencia a los implicados en el cruzamiento o la fusión de células.
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BCH-ORGA-SCBD-12110-5 Organismo Hordeum vulgare (Barley, HORVU)Cultivos
Hordeum vulgare L. (spring barley) cultivar Golden Promise
EN
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Spring barley with a mutation in the VRN1 gene (vrn1_LOF_4del)| Institute of Experimental Botany, AS CR(IEB) | Cambios en la fisiología y/o producción (Índice de crecimiento)
CPGE_VEC00113
EN
- Edición génica (por ej, CRISPR-Cas, etc.)
Algunos de estos elementos genéticos pueden estar presentes como fragmentos o formas truncadas. Por favor, véanse las notas a continuación, en los casos en los que corresponda.
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BCH-GENE-SCBD-299561-1 VRN1 | Hordeum vulgare (Barley, HORVU)Secuencia de codificación de proteínas | Cambios en la fisiología y/o producción (Índice de crecimiento, Reproducción, Tecnología de restricción de uso genético (TRUG), Esterilidad masculina)
Information on the inserted DNA sequence
The vrn1_LOF_76del barley line was generated using CRISPR/Cas-mediated genome editing following Agrobacterium-mediated transformation with the 18,084 bp vector CPGE_VEC00113.
The vrn1_LOF_76del barley line was generated using CRISPR/Cas-mediated genome editing following Agrobacterium-mediated transformation with the 18,084 bp vector CPGE_VEC00113.
The retained genetic modification consists of a 76 bp deletion in the first exon of the endogenous HvVRN1 (vernalization1) gene. The deletion disrupts the reading frame, preventing production of a functional HvVRN1 protein and resulting in a loss-of-function allele. HvVRN1 encodes an APETALA1/FRUITFULL-like MADS-box transcription factor that functions as a key regulator of vernalization responses and flowering in barley. The modified sequence is derived entirely from the endogenous barley VRN1 locus and does not introduce a novel coding sequence.
CPGE_VEC00113 contained Cas9, two guide RNA expression units targeting HvVRN1, the hpt selectable marker for transformed plant cells, the aadA bacterial selectable marker, and associated regulatory sequences. Following transformation and genome editing, regenerated plants were self-pollinated and progeny in which the vector-derived transgenic sequences had segregated away were selected. Their absence was repeatedly confirmed by molecular analyses in the T1 and T2 generations.
The T3 vrn1_LOF_76del plants selected for environmental release are homozygous for the 76 bp deletion and contain no introduced foreign genetic material.
Note
- The 76 bp deletion in the HvVRN1 locus was confirmed by Sanger sequencing.
- PCR and droplet digital PCR (ddPCR) were used to confirm the absence of vector-derived transgenic sequences in the T1 and T2 generations.
- CPGE_VEC00113 was used during development of the line but is not present in the final T3 plants.
- No foreign DNA or novel coding sequence is present in the final vrn1_LOF_76del line.
ES
- Investigación
ES
HvVRN1 is an important determinant of flowering time and adaptation of barley to different climatic conditions and growing seasons. The vrn1_LOF_76del line was developed to investigate under field conditions the effects of HvVRN1 loss of function on flowering, vernalization responses, environmental adaptation and agronomic performance. The evaluation includes phenological and agronomic traits such as heading date, tillering, yield-related characteristics and grain quality parameters.
Compared with non-modified control plants, vrn1_LOF_76del plants have been reported to flower approximately 38 days later and to exhibit increased tillering.
- A set of GMO spring barley lines with a mutation in the VRN1 gene for testing potential agricultural applications [ English ]
- Large deletions within the first intron in VRN-1 are associated with spring growth habit in barley and wheat [ English ]
- Direct links between the vernalization response and other key traits of cereal crops [DOI] [ English ]
- European Commission Food and Feed Information Portal. POL-GMOB-13842 - Spring barley with a mutation in the VRN1 gene [URL] [ English ]
- Large deletions within the first intron in VRN-1 are associated with spring growth habit in barley and wheat [DOI] [ English ]
- Development of the modular CasCADE vector system and its use for targeted mutagenesis [ Alemán ]
- Plant Physiology (2008). Low-Temperature and Daylength Cues Are Integrated to Regulate FLOWERING LOCUS T in Barley [PDF] ( barley, flowering, vernalization, HvVRN ) [ English ]
| Tipo de registro | Campo | Registro(s) | |
|---|---|---|---|
| Evaluación del riesgo generada por un proceso regulatorio | Organismo(s) vivo(s) modificado(s) | 1 | |
| Organismos vivos modificados | OVM relacionado(s) | 1 | |
