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Organisme vivant modifié
(LMO)
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Roundup Ready™ cotton
EN
1698
Oui
MON-89383-1
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Organisation :Monsanto ()800 North Lindbergh Blvd.St. Louis, MO
63167, États-Unis d'AmériqueTéléphone : + 1 314 694-1000,Télécopieur : +1 314 694-3080,E-mail :Site Web : http://www.monsanto.com,
Cotton tolerant to the herbicide glyphosate through incorporation of the epsps gene encoding 5-enolpyruvylshikimaete-3-phosphate synthase (EPSPS), that confers tolerance to the herbicide glyphosate. The neomycin phosphotransferase II (npt II) gene confers resistance to the antibiotic kanamycin.
EN
Le terme « organisme récepteur » désigne un organisme (non modifié ou déjà modifié) ayant fait l'objet d'une modification génétique, tandis que les « organismes parentaux » désignent les organismes impliqués dans un croisement ou une fusion cellulaire pour générer un organisme modifié.
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BCH-ORGA-SCBD-12080-6 Organisme Gossypium hirsutum (Cotton)Cultures
Variety: Coker 312
EN
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MON-Ø1445-2 - Roundup Ready™ cotton| Monsanto | Résistance aux antibiotiques (Kanamycine, Streptomycine), Résistance aux herbicides (Glyphosate)
PV-GHGT06
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- Transgénèse par agrobacterium
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Certains de ces éléments génétiques peuvent être présents sous forme de fragments ou sous forme tronquée. Veuillez consulter les notes ci-dessous, s’il y a lieu.
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BCH-GENE-SCBD-14979-7 5-enolpyruvylshikimate-3-phosphate synthase gene | Agrobacterium tumefaciens (Agrobacterium)Séquence codante de protéines | Résistance aux herbicides (Glyphosate)
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BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)Séquence codante de protéines | Résistance aux antibiotiques (Kanamycine)
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BCH-GENE-SCBD-101507-5 FMV 34S promoter | Figwort mosaic virus (Figwort mottle virus, FMV, CMoVb)Promoteur
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BCH-GENE-SCBD-100365-6 Chloroplast transit peptide 2 | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)Séquence signal
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BCH-GENE-SCBD-101877-5 rbcS-E9 gene terminator | Pisum sativum (Garden pea, PEA)Terminateur
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BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)Promoteur
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BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminateur
Information on the inserted DNA sequences
Molecular characterization demonstrates that one T-DNA (transferred) insert was integrated into the cotton genome to produce cotton 1698. The insert contains the CoMV promoter region, cp4epsps, nptII, aad (that is not expressed in plants) and a portion of ori-V coding regions. The cp4epsps gene encodes the naturally glyphosate tolerant EPSPS protein, the target site for glyphosate action. The nptII gene which is isolated from the Tn5 transposon encodes neomycin phosphotranferase type II (NPTII) which confers resistance to the aminoglycoside antibiotic kanamycin and neomycin. The nptII gene functions as a selectable marker and is driven by Cauliflower Mosaic Virus 35S promoter and is followed by a nopaline synthase (nos) 3' region which directs polyadenylation of the messenger RNA (mRNA). The aad gene encodes the bacterial selectable marker enzyme 3"(9) -O-aminoglycoside adenyltransferase (AAD) which allows for the selection of the bacteria containing the PV-GHGT06 to grow on medium with spectinomycin or streptomycin. This aad gene is not expressed in Roundup Ready 1698.
Vector information
The plasmid vector PV-GHGT06 contains well characterized DNA segments required for selection and replication of the plasmid in the bacteria as well as a right border for initiating the region of T-DNA, into the plant genomic DNA. The plant expression vectors were assembled, transformed in E. coli and mated into the ABI Agrobacterium strain by the triparental conjugation system using the helper plasmid pRK2013. The binary ABI strain contains the disarmed (lacking the T-DNA phytohormone genes) pTiC58 plasmid pMP90RK in a choramphenicol-resistant derivative of the A. tumefaciens strain A208. The disarmed pMP90RK Ti plasmid does not carry the T-DNA phytohormone genes and is unable to grow the crown gall disease.
EN
Molecular characterization demonstrates that one T-DNA (transferred) insert was integrated into the cotton genome to produce cotton 1698. The insert contains the CoMV promoter region, cp4epsps, nptII, aad (that is not expressed in plants) and a portion of ori-V coding regions. The cp4epsps gene encodes the naturally glyphosate tolerant EPSPS protein, the target site for glyphosate action. The nptII gene which is isolated from the Tn5 transposon encodes neomycin phosphotranferase type II (NPTII) which confers resistance to the aminoglycoside antibiotic kanamycin and neomycin. The nptII gene functions as a selectable marker and is driven by Cauliflower Mosaic Virus 35S promoter and is followed by a nopaline synthase (nos) 3' region which directs polyadenylation of the messenger RNA (mRNA). The aad gene encodes the bacterial selectable marker enzyme 3"(9) -O-aminoglycoside adenyltransferase (AAD) which allows for the selection of the bacteria containing the PV-GHGT06 to grow on medium with spectinomycin or streptomycin. This aad gene is not expressed in Roundup Ready 1698.
Vector information
The plasmid vector PV-GHGT06 contains well characterized DNA segments required for selection and replication of the plasmid in the bacteria as well as a right border for initiating the region of T-DNA, into the plant genomic DNA. The plant expression vectors were assembled, transformed in E. coli and mated into the ABI Agrobacterium strain by the triparental conjugation system using the helper plasmid pRK2013. The binary ABI strain contains the disarmed (lacking the T-DNA phytohormone genes) pTiC58 plasmid pMP90RK in a choramphenicol-resistant derivative of the A. tumefaciens strain A208. The disarmed pMP90RK Ti plasmid does not carry the T-DNA phytohormone genes and is unable to grow the crown gall disease.
FR
- Nourriture
- Nourriture pour les animaux
- Fibre / textile
Southern blot analysis indicated that there is a single locus into which the T-DNA integrated and that DNA contain 2 copies of the EPSPS coding sequence
EN
Cotton line 1698 was genetically engineered to express resistance to glyphosate, the active ingredient in the herbicide Roundup®, allowing for its use as a weed control option. In order to obtain field tolerance to glyphosate herbicide, a bacterial gene encoding a glyphosate-tolerant form of the enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) was introduced into the cotton genome by Agrobacterium-mediated transformation.
The EPSPS enzyme is present in all plants, bacteria, and fungi, and is part of an important biochemical pathway called the shikimate pathway, which is involved in the production of aromatic amino acids and other aromatic compounds. When conventional cotton plants are treated with glyphosate, the plants cannot produce the aromatic amino acids needed to grow and survive. EPSPS is not present in mammals, birds or aquatic life forms, which do not synthesize their own aromatic amino acids. For this reason, glyphosate has little toxicity to these organisms. The EPSPS enzyme is naturally present in foods derived from plant and microbial sources.
EN
The EPSPS enzyme is present in all plants, bacteria, and fungi, and is part of an important biochemical pathway called the shikimate pathway, which is involved in the production of aromatic amino acids and other aromatic compounds. When conventional cotton plants are treated with glyphosate, the plants cannot produce the aromatic amino acids needed to grow and survive. EPSPS is not present in mammals, birds or aquatic life forms, which do not synthesize their own aromatic amino acids. For this reason, glyphosate has little toxicity to these organisms. The EPSPS enzyme is naturally present in foods derived from plant and microbial sources.
| Type de fichier | Champs | Fichier(s) : | |
|---|---|---|---|
| Décision de pays ou tout autre communication | Identification de l'OVM | 2 | |
| Évaluation des risques générée par un processus de réglementation | Organisme(s) vivant(s) modifié(s) | 2 | |
| Organisme vivant modifié | OVM connexe(s) | 1 | |
