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Organisme vivant modifié
(LMO)
L’image ci-dessous identifie l’OVM par son identificateur unique, son nom commercial et un lien vers cette page du Centre d’échange Cliquer dessus pour télécharger une plus grande image sur votre ordinateur Pour du soutien sur comment utiliser, aller dans la page de liens rapides OVM.
Drought-tolerant, herbicide-tolerant, insect-resistant maize
EN
MON87460 × TC1507
Oui
MON-8746Ø-4 × DAS-Ø15Ø7-1
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Personne :Bayer CropScienceBayer CropScience AG Alfred-Nobel-Str. 50 40789 Monheim am RheinMonheim am Rhein,
40789, AllemagneTéléphone : +49 21 73 - 38-0,Télécopieur :E-mail :Organisations reliéesBayer CropScience Deutschland GmbH ()Secteur privé (affaires et industrie)Bayer CropScience AG Alfred-Nobel-Str. 50 40789 Monheim am RheinMonheim am Rhein,
40789, AllemagneTéléphone : +49 21 73 - 38-0,Télécopieur :E-mail : -
Personne :Dow AgroSciences GmbHTruderinger Straße 15München, Bayern
81677, AllemagneTéléphone : + 49 89 - 4 55 33 - 0,Télécopieur : + 49 89 - 4 55 33 - 111,E-mail : DowAgroSciencesD@dow.com,Site Web : http://www.dowagro.com/de,Organisations reliéesDow AgroSciences GmbH ()Secteur privé (affaires et industrie)Truderinger Straße 15München, Bayern
81677, AllemagneTéléphone : + 49 89 - 4 55 33 - 0,Télécopieur : + 49 89 - 4 55 33 - 111,E-mail : DowAgroSciencesD@dow.com,Site Web : http://www.dowagro.com/de,
The maize (Zea mays) was produced through cross breeding of modified parental maize lines for drought tolerance, herbicide tolerance and insect resistance. For abiotic tolerance, the maize expresses Bacillus subtillus cold shock protein to enhance natural abiotic (drought) stress responses. For herbicide tolerance, the maize expresses Streptomyces viridochromogenes phosphinothricin N-acetyltransferase, which inactivates glufosinate through acetyalation. For Lepidoptera tolerance, the maize expresses Bacillus thuringiensis Cry1F. Additionally, the maize contains an Escherichia coli neomycin phosphotransferase II cassette for kanamycin selection, which was used during transformation of a parental line.
EN
Le terme « organisme récepteur » désigne un organisme (non modifié ou déjà modifié) ayant fait l'objet d'une modification génétique, tandis que les « organismes parentaux » désignent les organismes impliqués dans un croisement ou une fusion cellulaire pour générer un organisme modifié.
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BCH-ORGA-SCBD-246-6 Organisme Zea mays (Maize, Corn, MAIZE)Cultures
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BCH-LMO-SCBD-103066-6 Organisme vivant modifié MON-8746Ø-4 - Droughtgard™ MaizeRésistance aux antibiotiques (Kanamycine, Néomycine), Tolérance au stress abiotique (Froid / chaleur, Sécheresse)
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BCH-LMO-SCBD-14841-16 Organisme vivant modifié DAS-Ø15Ø7-1 - Herculex™ I maizeDow AgroSciences, Pioneer Hi-Bred International Inc. | Résistance aux maladies et aux parasites (Insectes, Chenille tisseuse (papillons et mites), Pyrale du maïs (Ostrinia nubilalis)), Résistance aux herbicides (Glufosinate)
FR
PV-ZMAP595; PHI8999A
EN
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Certains de ces éléments génétiques peuvent être présents sous forme de fragments ou sous forme tronquée. Veuillez consulter les notes ci-dessous, s’il y a lieu.
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BCH-GENE-SCBD-101416-7 Ti plasmid right border repeat | Agrobacterium tumefaciens (Agrobacterium)Plasmid vector
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BCH-GENE-SCBD-100364-5 Rice actin 1 gene promoter | Oryza sativa (Rice, ORYSA)Promoteur
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BCH-GENE-SCBD-100355-6 Rice actin 1, intron | Oryza sativa (Rice, ORYSA)Intron
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BCH-GENE-SCBD-103065-7 Cold shock protein gene | Bacillus subtilis (Bacillus, BACIU)Séquence codante de protéines | Tolérance au stress abiotique (Froid / chaleur, Sécheresse)
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BCH-GENE-SCBD-103067-9 Transcript 7 gene 3' untranslated region | Agrobacterium tumefaciens (Agrobacterium)Terminateur
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BCH-GENE-SCBD-103069-3 loxP recombination site | Bacteriophage P1 (Phage P1)recombination site
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BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)Promoteur
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BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)Séquence codante de protéines | Résistance aux antibiotiques (Kanamycine)
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BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminateur
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BCH-GENE-SCBD-101415-9 Ti plasmid left border repeat | Agrobacterium tumefaciens (Agrobacterium)Plasmid vector
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BCH-GENE-SCBD-100362-7 Ubiquitin gene promoter | Zea mays (Maize, Corn, MAIZE)Promoteur
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BCH-GENE-SCBD-103627-5 Ubiquitin Intron 1 | Zea mays (Maize, Corn, MAIZE)Intron
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BCH-GENE-SCBD-14987-8 Cry1F | Bacillus thuringiensis (Bt, Bacillus, BACTU)Séquence codante de protéines | Résistance aux maladies et aux parasites (Insectes, Chenille tisseuse (papillons et mites))
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BCH-GENE-SCBD-100363-5 ORF25 PolyA Terminator sequence | Agrobacterium tumefaciens (Agrobacterium)Terminateur
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BCH-GENE-SCBD-15002-5 Phosphinothricin N-acetyltransferase | Streptomyces viridochromogenes (STRVR)Séquence codante de protéines | Résistance aux herbicides (Glufosinate)
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BCH-GENE-SCBD-100290-6 CaMV 35S terminator | Cauliflower mosaic virus (CaMV)Terminateur
DNA insert from MON87460 vector PV-ZMAP595
The T-DNA insert contains the following gene cassettes: Bacillus subtillus cold shock protein (cspB) and Escherichia coli neomycin phosphotransferase II (nptII).
Transcription of cspB is under control of the Oryza sativa actin 1 promoter and Agrobacterium tumefaciens transcript 7 gene 3' untranslated region. The transcript initially contains an O. sativa actin 1 intron for enhanced gene expression of cspB. The sequence is removed (spliced) prior to protein translation. Constitutive expression of cspB is expected due to the actin promoter.
Transcription of nptII is under control of the Cauliflower mosaic virus (CaMV) 35S promoter and A. tumefaciens nopaline synthase terminator. High levels of transcription are expected due to the CaMV promoter.
Note:
- The coding sequence of cspB has been codon optimized for optimal expression within plant cells.
- Southern blot analysis indicated that no vector backbone sequences were inserted into the parental genome
- Southern blot analysis indicated that the parental genome contains a single insertion
- Sequencing analyses confirm the Southern blot analyses.
- A 22 base pair deletion of genomic DNA at the insert-to-plant DNA junction occurred.
- loxP sites can be found in the parental genome and could potentially allow for the excision of the nptII cassette by CRE recombinase.
DNA insert from TC1507 vector PHI8999A
DNA fragment PHI8999A contains two adjacent plant gene expression cassettes for Bacillus thuringiensis cry1F and Streptomyces viridochromogenes pat.
Transcription of cry1F is directed by the promoter and first exon and intron of the maize (Zea mays) ubiquitin gene and terminates at the Agrobacterium tumefaciens ORF25 terminator.
Transcription of the pat gene commences from the Cauliflower mosaic virus (CaMV) 35S promoter and ends at the CaMV 35S terminator.
Note:
- The coding sequence of both genes has been optimized to achieve a high level of expression in maize.
- The sequences of the complete cry1F and pat are identical to those in the original plasmid.
- The CRY1F protein includes the F604K (phenylalanine to lysine at position 604) amino acid substitution, which was introduced to create a specific restriction site for cloning purposes.
For more information, kindly refer to the parental LMO records.
EN
The T-DNA insert contains the following gene cassettes: Bacillus subtillus cold shock protein (cspB) and Escherichia coli neomycin phosphotransferase II (nptII).
Transcription of cspB is under control of the Oryza sativa actin 1 promoter and Agrobacterium tumefaciens transcript 7 gene 3' untranslated region. The transcript initially contains an O. sativa actin 1 intron for enhanced gene expression of cspB. The sequence is removed (spliced) prior to protein translation. Constitutive expression of cspB is expected due to the actin promoter.
Transcription of nptII is under control of the Cauliflower mosaic virus (CaMV) 35S promoter and A. tumefaciens nopaline synthase terminator. High levels of transcription are expected due to the CaMV promoter.
Note:
- The coding sequence of cspB has been codon optimized for optimal expression within plant cells.
- Southern blot analysis indicated that no vector backbone sequences were inserted into the parental genome
- Southern blot analysis indicated that the parental genome contains a single insertion
- Sequencing analyses confirm the Southern blot analyses.
- A 22 base pair deletion of genomic DNA at the insert-to-plant DNA junction occurred.
- loxP sites can be found in the parental genome and could potentially allow for the excision of the nptII cassette by CRE recombinase.
DNA insert from TC1507 vector PHI8999A
DNA fragment PHI8999A contains two adjacent plant gene expression cassettes for Bacillus thuringiensis cry1F and Streptomyces viridochromogenes pat.
Transcription of cry1F is directed by the promoter and first exon and intron of the maize (Zea mays) ubiquitin gene and terminates at the Agrobacterium tumefaciens ORF25 terminator.
Transcription of the pat gene commences from the Cauliflower mosaic virus (CaMV) 35S promoter and ends at the CaMV 35S terminator.
Note:
- The coding sequence of both genes has been optimized to achieve a high level of expression in maize.
- The sequences of the complete cry1F and pat are identical to those in the original plasmid.
- The CRY1F protein includes the F604K (phenylalanine to lysine at position 604) amino acid substitution, which was introduced to create a specific restriction site for cloning purposes.
For more information, kindly refer to the parental LMO records.
FR
- Nourriture
- Nourriture pour les animaux
- MON-8746Ø-4 - EU Reference Laboratory for GM Food and Feed (EURL-GMFF) [ English ]
- DAS-Ø15Ø7-1 - EU Reference Laboratory for GM Food and Feed (EURL-GMFF) [ English ]
- DAS-Ø15Ø7-1 - EU Reference Laboratory for GM Food and Feed (EURL-GMFF) ( JRC ) [ English ]
- MON-8746Ø-4 - EU Reference Laboratory for GM Food and Feed (EURL-GMFF) ( JRC ) [ English ]
- MON-8746Ø-4 - CropLife International Detection Methods Database ( CropLife ) [ English ]
- DAS-Ø15Ø7-1 - CropLife International Detection Methods Database ( CropLife ) [ English ]
FR
FR
- EUginius - MON87460 x DAS1507 [ English ]
| Type de fichier | Champs | Fichier(s) : | |
|---|---|---|---|
| Décision de pays ou tout autre communication | Identification de l'OVM | 2 | |
| Évaluation des risques générée par un processus de réglementation | Organisme(s) vivant(s) modifié(s) | 2 | |
| Laboratoire pour la détection et d'identification des OVM | OVM(s) détectable(s) par le laboratoire | 1 | |
