Loading...
Cet enregistrement a été supprimé. Comme ce document est lié à un enregistrement publié, il est disponible ici à titre de référence uniquement.
Cliquez ici pour consulter l'enregistrement suppriméCe document a été mis à jour. Il ne s’agit pas de la dernière version publiée. Cliquez ici pour voir la dernière version du fichier.
Organisme vivant modifié
(LMO)
L’image ci-dessous identifie l’OVM par son identificateur unique, son nom commercial et un lien vers cette page du Centre d’échange Cliquer dessus pour télécharger une plus grande image sur votre ordinateur Pour du soutien sur comment utiliser, aller dans la page de liens rapides OVM.
Herbicide tolerant soybean
EN
MON94313
Oui
MON-94313-8
-
Organisation :Bayer CropScience ()Téléphone :Télécopieur :E-mail :Site Web : http://www.bayercropscience.com,
Soybean (Glycine max) was modified to confer tolerance to multiple herbicides. For resistance to 2-methoxy-3,6-dichlorobenzoic acid (dicamba), the soybean expresses Stenotrophomonas maltophilia dicamba monooxygenase, which degrades the herbicidal compound through oxygenation. For tolerance to glufosinate, the soybean expresses Streptomyces viridochromogenes phosphinothricin N-acetyltransferase, which inactivates the herbicidal compound through acetylation. For tolerance to quizalofop and 2,4-dichlorophenoxyacetic acid herbicides, the soybean expresses Sphingobium herbicidovorans R-2,4-dichlorophenoxypropionate dioxygenase, which inactivates the herbicides through oxygenation. The enzyme may also act on other aryloxyphenoxypropionate and synthetic auxin herbicides as well. For tolerance to β-triketone herbicides (e.g., mesotrione and benzobicyclon), the soybean expresses Oryza sativa triketone dioxygenase, which catalyzes hydroxylation of the herbicidal compounds to inactivate them. The modified soybean does not contain any selectable markers (splA and aadA expression cassettes) as they were crossed out of the line during development of the commercial line.
Le terme « organisme récepteur » désigne un organisme (non modifié ou déjà modifié) ayant fait l'objet d'une modification génétique, tandis que les « organismes parentaux » désignent les organismes impliqués dans un croisement ou une fusion cellulaire pour générer un organisme modifié.
-
BCH-ORGA-SCBD-10453-8 Organisme Glycine max (Soybean, Soya bean, Soya / soja /соя, SOYBN)Cultures
Soybean variety A3555
EN
PV-GMHT529103
EN
- Transgénèse par agrobacterium
|
1.008 kb
|
|
0.204 kb
|
|
1.023 kb
|
|
0.500 kb
|
|
0.409 kb
|
|
0.309 kb
|
|
0.552 kb
|
|
0.500 kb
|
|
1.202 kb
|
|
0.888 kb
|
|
0.500 kb
|
|
0.500 kb
|
|
0.300 kb
|
|
1.056 kb
|
|
0.300 kb
|
Certains de ces éléments génétiques peuvent être présents sous forme de fragments ou sous forme tronquée. Veuillez consulter les notes ci-dessous, s’il y a lieu.
-
BCH-GENE-SCBD-272454-1 Ubiquitin 3 promoter | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)Promoteur
-
BCH-GENE-SCBD-115846-2 Albino and pale green 6 target sequence | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)Séquence signal
-
BCH-GENE-SCBD-100728-3 Dicamba monooxygenase gene | Stenotrophomonas maltophilia (S. maltophilia, Stenotrophomonas)Séquence codante de protéines | Résistance aux herbicides
-
BCH-GENE-SCBD-272455-1 Sali3-2 BURP domain protein terminator | Medicago truncatula (Barrelclover, Strong-spined medick, Barrel medic, Barrel medick, MEDTR)Terminateur
-
BCH-GENE-SCBD-272456-1 Synthetic promoter GSP579 | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)Promoteur
-
BCH-GENE-SCBD-272457-1 Synthetic intron GSI102 | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)Intron
-
BCH-GENE-SCBD-15002-5 Phosphinothricin N-acetyltransferase | Streptomyces viridochromogenes (STRVR)Séquence codante de protéines | Résistance aux herbicides (Glufosinate)
-
BCH-GENE-SCBD-272458-1 Heat shock protein 20 terminator | Medicago truncatula (Barrelclover, Strong-spined medick, Barrel medic, Barrel medick, MEDTR)Terminateur
-
BCH-GENE-SCBD-104802-5 Polyubiquitin10 gene promoter | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)Promoteur
-
BCH-GENE-SCBD-115849-2 R-2,4-dichlorophenoxypropionate dioxygenase | Sphingobium herbicidovorans (SPHHE)Séquence codante de protéines | Résistance aux herbicides,Tolerance to 2,4-dichlorophenoxyacetic acid,Tolerance to quizalofop
-
BCH-GENE-SCBD-272459-1 Gene of unknown function 1 terminator | Medicago truncatula (Barrelclover, Strong-spined medick, Barrel medic, Barrel medick, MEDTR)Terminateur
-
BCH-GENE-SCBD-272460-1 Triketone dioxygenase | Oryza sativa (Rice, ORYSA)Séquence codante de protéines | Résistance aux herbicides
-
BCH-GENE-SCBD-272461-1 Synthetic terminator GST7 | Zea mays (Maize, Corn, MAIZE)Terminateur
The modified soybean contains four gene cassettes: Stenotrophomonas maltophilia dicamba monooxygenase (dmo), Streptomyces viridochromogenes phosphinothricin N-acetyltransferase gene (pat), Sphingobium herbicidovorans R-2,4-dichlorophenoxypropionate dioxygenase (ft_t.1) and Oryza sativa triketone dioxygenase (tdo).
Transcription of dmo is under the control of an Arabidopsis thaliana ubiquitin 3 promoter and a Medicago truncatula sali3-2 protein terminator. An A. thaliana albino and pale green 6 target peptide was included to direct the translated protein to the chloroplast. Due to the constitutive nature of the promoter, high levels of transcription are expected.
Transcription of pat is under control of a synthetic promoter GSP579 and a M. truncatula heat shock protein 20 terminator. A synthetic intron (GI102) was included to enhance expression of the pat sequence. Due to the constitutive nature of the promoter, high levels of transcription are expected.
Transcription of ft_t.1 is under control of an A. thaliana ubiquitin 10 promoter and a M. truncatula gene of unknown function 1 terminator. Due to the constitutive nature of the promoter, high levels of transcription are expected.
Transcription of tdo is under control of a synthetic promoter GSP579, a synthetic intron GSI102 and a synthetic terminator GST7. Given the constitutive nature of promoter and enhancement by the other synthetic regulatory elements, high levels of expression are expected.
Note:
Transcription of dmo is under the control of an Arabidopsis thaliana ubiquitin 3 promoter and a Medicago truncatula sali3-2 protein terminator. An A. thaliana albino and pale green 6 target peptide was included to direct the translated protein to the chloroplast. Due to the constitutive nature of the promoter, high levels of transcription are expected.
Transcription of pat is under control of a synthetic promoter GSP579 and a M. truncatula heat shock protein 20 terminator. A synthetic intron (GI102) was included to enhance expression of the pat sequence. Due to the constitutive nature of the promoter, high levels of transcription are expected.
Transcription of ft_t.1 is under control of an A. thaliana ubiquitin 10 promoter and a M. truncatula gene of unknown function 1 terminator. Due to the constitutive nature of the promoter, high levels of transcription are expected.
Transcription of tdo is under control of a synthetic promoter GSP579, a synthetic intron GSI102 and a synthetic terminator GST7. Given the constitutive nature of promoter and enhancement by the other synthetic regulatory elements, high levels of expression are expected.
Note:
- The ubiquitin 10 promoter contains leader and intron sequences.
- The tdo sequence was codon-optimized for expression in soybean.
- Next-generation sequence analyses indicated that the line contains a single T-DNA insert without integration of vector backbone sequences. Directed sequence analyses indicated that the genetic cassettes were presented as expected (without rearrangement).
- A second T-DNA was inserted during the production of MON94313. The second T-DNA contained selectable marker cassettes for 3'(9)-O-nucleotidyltransferase (aadA) and sucrose phosphorylase (splA). Subsequent segregation, selection and screening were used to isolate those plants that did not contain the second T-DNA insertion following the crossing of T1 generation progeny. See attached regulatory applications for more details.
FR
- Nourriture pour les animaux
- Nourriture
- MON-94313-8 - EU Reference Laboratory for GM Food and Feed (EURL-GMFF) ( JRC ) [ English ]
FR
FR
- EUginius - MON94313 [ English ]
- A1276 Application - MON 94313.pdf [ English ]
- APHIS-Request for a Regulatory Status Review for MON94313.pdf [ English ]
| Type de fichier | Champs | Fichier(s) : | |
|---|---|---|---|
| Décision de pays ou tout autre communication | Identification de l'OVM | 3 | |
| Évaluation des risques générée par un processus de réglementation | Organisme(s) vivant(s) modifié(s) | 5 | |
