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ddPCR strategy to detect a gene-edited plant carrying a single variation point: Technical feasibility and interpretation issues (Publication Date: Mar 2022)

Author's Abstract. Gene-edited organisms and derived food and feed products commercialized on the European market falls within the scope of the Directive, 2001/18/EC. Therefore, the possibility to specifically detect and quantify them has become a priority. To this end, PCR-based approaches, such as real-time PCR and digital droplet PCR, targeting a single variation point carried by a gene-edited organism are expected to be suitable, even if potentially challenging at the technical level. Howev ...

Biosafety Virtual Library Resources
bch-vlr-scbd-281901-2
Article
15 Jan 2026

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Detection and identification of authorized and unauthorized GMOs using high-throughput sequencing with the support of a sequence-based GMO database (Mar 2022)

The increasing number and diversity of genetically modified organisms (GMOs) for the food and feed market calls for the development of advanced methods for their detection and identification. This issue can be addressed by next generation sequencing (NGS). However, the efficiency of NGS-based strategies depends on the availability of bioinformatic methods to find sequences of the transgenic insert and junction regions, which is a challenging topic. To facilitate this task, we have developed Nexp ...

Biosafety Virtual Library Resources
bch-vlr-scbd-259918-2
Article
16 Apr 2025

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Nexplorer (Jan 2022)

Nexplorer is a database containing authorised GMO events and links to their respective annotated DNA sequences, which are stored in a structured, searchable and extractable format. It is made available as a web-application offering a user-friendly interface that allows non-IT experts to manage the database and the sequences. This application gives the GMO lab a secure way to store the data by the use of functionalities such as change tracking and confidentiality management.

Biosafety Virtual Library Resources
bch-vlr-scbd-259917-3
Online database
16 Apr 2025

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LAB - Sciensano

Other

Laboratory for detection and identification of LMOs
bch-lab-scbd-250598-2
Belgium
16 Apr 2025

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Royal Belgian Institute of Natural Sciences

The Royal Belgian Institute of Natural Sciences is natural science museum and a research institute.

Biosafety Organization
bch-org-scbd-15939-10
Government agency (National/Federal)
29 Mar 2021

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