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Living Modified Organism
(LMO)
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Agrisure™ RW Rootworm-Protected Roundup Ready™ maize
EN
MIR604 x GA21
Yes
SYN-IR6Ø4-5 × MON-ØØØ21-9
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Organization:Syngenta Crop Protection AG ()Schwarzwaldallee 215Basel,
CH 4058 , SwitzerlandPhone: +41 61 323-1111,Fax:Email:Website: http://www.syngenta.com,
SYN-IR6Ø4-5×MON-ØØØ21-9 has stacked insect resistant and herbicide tolerant maize produced by conventional cross breeding of two genetically engineered parental varieties (MIR604 and GA21).
The parental MIR604 maize variety (OECD unique identifier SYN-IR6Ø4-5) was genetically modified by introducing the cry3A gene from Bacillus thuringiensis ssp. kumamotoensis, which codes for a Bt-toxin (Cry3A) and confers resistance to western corn rootworm (Diabrotica virgifera virgifera), northern corn rootworm (Diabrotica longicornis barberi) and other related coleopteran species. Expression of the pmi gene in MIR604 from the bacterium Escherichia coli allows this line to use mannose as a carbon source through production of the PMI protein, and is used as a selectable marker.
The parental GA21 line (OECD unique identifier MON-ØØØ21-9) was created by modifying the endogenous maize epsps gene through site-directed mutagenesis, such that its encoded enzyme was insensitive to inactivation by glyphosate, and inserting it into the inbred AT maize variety. The modified maize GA21 line permits farmers to use glyphosate-containing herbicides for weed control in the cultivation of maize.
EN
The parental MIR604 maize variety (OECD unique identifier SYN-IR6Ø4-5) was genetically modified by introducing the cry3A gene from Bacillus thuringiensis ssp. kumamotoensis, which codes for a Bt-toxin (Cry3A) and confers resistance to western corn rootworm (Diabrotica virgifera virgifera), northern corn rootworm (Diabrotica longicornis barberi) and other related coleopteran species. Expression of the pmi gene in MIR604 from the bacterium Escherichia coli allows this line to use mannose as a carbon source through production of the PMI protein, and is used as a selectable marker.
The parental GA21 line (OECD unique identifier MON-ØØØ21-9) was created by modifying the endogenous maize epsps gene through site-directed mutagenesis, such that its encoded enzyme was insensitive to inactivation by glyphosate, and inserting it into the inbred AT maize variety. The modified maize GA21 line permits farmers to use glyphosate-containing herbicides for weed control in the cultivation of maize.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-246-6 Organism Zea mays (Maize, Corn, MAIZE)Crops
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BCH-LMO-SCBD-15105-12 Living Modified Organism SYN-IR6Ø4-5 - Agrisure™ RW Rootworm-Protected maizeResistance to diseases and pests (Insects, Coleoptera (beetles))
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BCH-LMO-SCBD-14794-18 Living Modified Organism MON-ØØØ21-9 - Roundup Ready™ maizeMonsanto | Resistance to herbicides (Glyphosate)
EN
pZM26 and pDPG434
EN
- Cross breeding
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-15003-7 Phosphomannose Isomerase gene | Escherichia coli (ECOLX)Protein coding sequence | Mannose tolerance,Selectable marker genes and reporter genes
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BCH-GENE-SCBD-100364-5 Rice actin 1 gene promoter | Oryza sativa (Rice, ORYSA)Promoter
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BCH-GENE-SCBD-100355-6 Rice actin 1, intron | Oryza sativa (Rice, ORYSA)Intron
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BCH-GENE-SCBD-101419-4 Optimized Transit PeptideTransit signal
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BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminator
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BCH-GENE-SCBD-103881-2 Metallothionein-like gene promoter | Zea mays (Maize, Corn, MAIZE)Promoter
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BCH-GENE-SCBD-100362-7 Ubiquitin gene promoter | Zea mays (Maize, Corn, MAIZE)Promoter
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BCH-GENE-SCBD-103627-5 Ubiquitin Intron 1 | Zea mays (Maize, Corn, MAIZE)Intron
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BCH-GENE-SCBD-43634-3 mCry3A | Bacillus thuringiensis (Bt, Bacillus, BACTU)Protein coding sequence | Resistance to diseases and pests (Insects, Coleoptera (beetles), Western corn rootworm (Diabrotica virgifera))
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BCH-GENE-SCBD-46333-8 5-enolpyruvylshikimate-3-phosphate synthase | Zea mays (Maize, Corn, MAIZE)Protein coding sequence | Resistance to herbicides (Glyphosate)
Inserted genes (MIR604):
1) cry3A-gene from Bacillus thuringiensis ssp. kumamotoensis. This gene codes for a Bt-toxin, which confers resistance to western corn rootworm (Diabrotica virgifera virgifera), northern corn rootworm (Diabrotica longicornis barberi) and other related coleopteran species.
2) Marker gene pmi-gene from the bacterium Escherichia coli. The LMO plant harboring this gene expresses a marker protein, PMI, that allows the plant to utilise mannose as a carbon source, acting as a selectable marker.
Inserted gene (GA21):
m epsps. This is a mutated form of the epsps gene derived from maize (Zea mays). The cp4 epsps gene encodes for a version of EPSPS that is highly tolerant to inhibition by glyphosate.
EN
1) cry3A-gene from Bacillus thuringiensis ssp. kumamotoensis. This gene codes for a Bt-toxin, which confers resistance to western corn rootworm (Diabrotica virgifera virgifera), northern corn rootworm (Diabrotica longicornis barberi) and other related coleopteran species.
2) Marker gene pmi-gene from the bacterium Escherichia coli. The LMO plant harboring this gene expresses a marker protein, PMI, that allows the plant to utilise mannose as a carbon source, acting as a selectable marker.
Inserted gene (GA21):
m epsps. This is a mutated form of the epsps gene derived from maize (Zea mays). The cp4 epsps gene encodes for a version of EPSPS that is highly tolerant to inhibition by glyphosate.
EN
- Food
- SYN-IR6Ø4-5 - EU Reference Laboratory for GM Food and Feed (EURL-GMFF) [ English ]
- MON-ØØØ21-9 - EU Reference Laboratory for GM Food and Feed (EURL-GMFF) [ English ]
- MON-ØØØ21-9 - EU Reference Laboratory for GM Food and Feed (EURL-GMFF) ( JRC ) [ English ]
- SYN-IR6Ø4-5 - EU Reference Laboratory for GM Food and Feed (EURL-GMFF) ( JRC ) [ English ]
- SYN-IR6Ø4-5 - CropLife International Detection Methods Database ( CropLife ) [ English ]
- MON-ØØØ21-9 - CropLife International Detection Methods Database ( CropLife ) [ English ]
EN
This plant is a product of traditional plant breeding, and therefore is not automatically subject to regulation in all jurisdictions as are transgenic plants resulting from recombinant DNA technologies. Certain jurisdictions may request notification in advance of the release of a stacked hybrid, or may request information to conduct an environmental and food safety assessment.
EN
- SYN-IR6Ø4-5 x MON ØØØ21-9 - GMO Compass [ English ]
- MIR604xGA21_application_food_feed.pdf [ English ]
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