BCS-GHØØ4-7 - Herbicide-tolerant, insect-resistant cotton | BCH-LMO-SCBD-101018 | Living Modified Organism | Biosafety Clearing-House


Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
published: 04 Mar 2010 last updated: 19 Jan 2023
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Herbicide-tolerant, insect-resistant cotton
The cotton (Gossypium hirsutum) was modified for insect-resistance and herbicide tolerance. To protect the cotton from feeding damage by Lepidopteran insect larvae, the cotton expresses a modified Bacillus thuringiensis Cry1Ab protein, a delta endotoxin, which forms pores in the midgut of the insect. For tolerance to herbicides containing glufosinate ammonium, the cotton expresses Streptomyces hygroscopicus phosphinothricin acetyltransferase, which inactivates the herbicidal compounds through acetylation.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
Cultivar Coker 315
Characteristics of the modification process
  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-14972-12 Phosphinothricin N-acetyltransferase gene | Streptomyces hygroscopicus (STRHY)
    Protein coding sequence | Resistance to herbicides (Glufosinate)
  • BCH-GENE-SCBD-14985-12 Cry1Ab | Bacillus thuringiensis (Bt, Bacillus, BACTU)
    Protein coding sequence | Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths))
  • BCH-GENE-SCBD-101021-3 Ps7s7 | Subterranean clover stunt virus (SCSV, Subterranean clover stunt virus)
  • BCH-GENE-SCBD-101025-5 NADP-malic enzyme 1 gene 3'UTR and terminator | Flaveria bidentis (Coastal plain yellowtops, FLABI)
  • BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)
  • BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)
  • BCH-GENE-SCBD-101416-6 Ti plasmid right border repeat | Agrobacterium tumefaciens (Agrobacterium)
    Plasmid vector
  • BCH-GENE-SCBD-101415-9 Ti plasmid left border repeat | Agrobacterium tumefaciens (Agrobacterium)
    Plasmid vector
  • BCH-GENE-SCBD-104947-3 5'e1 Leader | Oryza sativa (Rice, ORYSA)
The modified cotton contains two gene cassettes: Bacillus thuringiensis cry1Ab and Streptomyces hygroscopicus phosphinothricin N-acetyltransferase (bar).

The cry1Ab sequence is under control of a Subterranean clover stunt virus genome segment 7 promoter and Flaveria bidentis NADP-malic enzyme 1 (me1) 3' untranslated region. An Oryza sativa 5' E1 leader was also included to enhance the expression of cry1Ab.

The bar sequence is under control of a Cauliflower mosaic virus 35S promoter and Agrobacterium tumefaciens nopaline synthase terminator. 

  • The cry1Ab gene has been derived from Bacillus thuringiensis strain berliner 1715 (Genbank accession No. X04698 - first cloned and characterised by Höfte et al (1986) http://www.lifesci.sussex.ac.uk/home/Neil_Crickmore/Bt/toxins2.html).
  • Sequencing of the 9056 bp inserted transgenic construct and Southern blot analysis revealed an almost full copy of the T-DNA construct (with an incomplete me1 terminator) was inserted into the T304-40 LM cotton line in addition to:
    •  a partial 3’me1 terminator;
    • a partial copy of the cry1Ab gene cassette, with a truncated Ps7s7 promoter, in a tail-to-tail orientation; and
    • a partial copy of the bar gene cassette in which the nos terminator is truncated.
  • As a result of the transformation event, four new junctions were created (see Figure 4 in the attached file below), two being located at the 5’ and 3’ ends of the insert and two being located within the insert as a result of the rearrangement. 
  • Southern blot analysis revealed the event contains a single insert with no vector backbone sequences integrated into the T304-40 genome.
  • The vector pTDL008 was derived from pGSV20.
LMO characteristics
  • Feed
  • Fiber/textile
  • Food
  • Other (Industrial)
Detection method(s)
Additional Information