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Living Modified Organism (LMO)
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BCH-LMO-SCBD-101018-15   |   PDF   |   Print   |  
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Decisions on the LMO Risk Assessments  
published: 04 Mar 2010 last updated: 13 Jul 2026
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Herbicide-tolerant, insect-resistant cotton
EN
T304-40
Yes
BCS-GHØØ4-7
The cotton (Gossypium hirsutum) was modified for resistance against lepidopteran insect pests and glufosinate-ammonium tolerance. To achieve resistance against Lepidoptera, the cotton expresses the cry1Ab gene from Bacillus thuringiensis subsp. kurstaki, which produces a crystal delta-endotoxin that binds to specific midgut receptors in the insect larva, forming pores that disrupt osmotic balance and cause fatal cell lysis. To achieve glufosinate tolerance, the cotton expresses Streptomyces hygroscopicus phosphinothricin N-acetyltransferase encoding pat gene, which inactivates the active compound L-phosphinothricin through acetylation of the primary amino group.
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
Cultivar Coker 315
EN
Characteristics of the modification process
pTDL008 derived from pGSV20
EN
  • Agrobacterium-mediated DNA transfer
 
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0.940 kb
 
 
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1.040 kb
 
 
0.860 kb
 
 
0.550 kb
 
 
0.310 kb
 
 
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-14972-12 Phosphinothricin N-acetyltransferase gene | Streptomyces hygroscopicus (STRHY)
    Protein coding sequence | Resistance to herbicides (Glufosinate)
  • BCH-GENE-SCBD-14985-12 Cry1Ab | Bacillus thuringiensis (Bt, Bacillus, BACTU)
    Protein coding sequence | Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths))
  • BCH-GENE-SCBD-101021-3 Ps7s7 | Subterranean clover stunt virus (SCSV, Subterranean clover stunt virus)
    Promoter
  • BCH-GENE-SCBD-101025-5 NADP-malic enzyme 1 gene 3'UTR and terminator | Flaveria bidentis (Coastal plain yellowtops, FLABI)
    Terminator
  • BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)
    Promoter
  • BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)
    Terminator
  • BCH-GENE-SCBD-101416-7 Ti plasmid right border repeat | Agrobacterium tumefaciens (Agrobacterium)
    Plasmid vector
  • BCH-GENE-SCBD-101415-9 Ti plasmid left border repeat | Agrobacterium tumefaciens (Agrobacterium)
    Plasmid vector
  • BCH-GENE-SCBD-104947-3 5'e1 Leader | Oryza sativa (Rice, ORYSA)
    Leader
Information on the inserted DNA sequences
The transforming plasmid pTDL008 contains two gene cassettes: Bacillus thuringiensis crystal protein cry1Ab and Streptomyces hygroscopicus phosphinothricin N-acetyltransferase (bar).

(1) The cry1Ab coding sequence is under the control of Subterranean clover stunt virus genome segment 7 (Ps7s7) promoter and Flaveria bidentis NADP-malic enzyme 1 terminator. The 5' leader sequence from Oryza sativa E1 gene was also included in the cassette to enhance the expression of cry1Ab. High levels of transcription is expected  due to the viral promoter.

(2) The bar coding sequence is under the control of a Cauliflower mosaic virus 35S promoter and Agrobacterium tumefaciens nopaline synthase terminator. High levels of transcription is expected  due to the viral promoter.

Note
  • The cry1Ab gene has been derived from Bacillus thuringiensis strain berliner 1715 (Genbank accession No. X04698 - first cloned and characterised by Höfte et al (1986)).
  • Sequencing of the 9056 bp inserted transgenic construct and Southern blot analysis revealed a truncated T-DNA construct (with an incomplete me1 terminator) was inserted into the T304-40 LM cotton line in addition to: (1) a partial 3’me1 terminator; (2) a partial copy of the cry1Ab gene cassette, with a truncated Ps7s7 promoter, in a tail-to-tail orientation; (3) a partial copy of the bar gene cassette in which the nos terminator is truncated.
  • As a result of the transformation event, four new junctions were created (see Figure 4 in the attached file below), two being located at the 5’ and 3’ ends of the insert and two being located within the insert as a result of the rearrangement. 
  • Southern blot analysis of the genomic DNA from COT102 revealed the incorporation of single intact copies of the vip3Aa and hpt genes, along with intact copies of their respective regulatory sequences. Results of these analyses also demonstrated that none of the vector backbone sequences, including the streptomycin adenyltransferase (aadA) gene, were incorporated into the genomic DNA.
EN
LMO characteristics
EN
  • Feed
  • Fiber/textile
  • Food
  • Other (Industrial)
Detection method(s)
EN
Records referencing this document Show in search
Record type Field Record(s)
Living Modified Organism Recipient Organism or Parental Organisms 8
Living Modified Organism Related LMO(s) 1
Country's Decision or any other Communication LMO identification 15
Risk Assessment generated by a regulatory process Living modified organism(s) 14
Laboratory for detection and identification of LMOs LMO(s) detectable by the laboratory 3

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