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Living Modified Organism
(LMO)
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Potato modified for the production of spider silk protein
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Solanum tuberosum pCB301-Kan-MaSpII-100xELP - 3 lines: 26, 27, 28
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- Organization: Leibniz Institute of Plant Genetics and Crop Plant Research (IPK) | BCH-CON-DE-49376-2Organization:Leibniz Institute of Plant Genetics and Crop Plant Research (IPK)Academic or research instituteCorrensstrasse 3Gatersleben,
06466 , GermanyPhone: +49 (0)39482 5-0,Fax: +49 (0)39482 5139,Email: info@ipk-gatersleben.de,
Potatoes pCB301-Kan-MaSpII-100xELP produce a fusion protein consisting of the spider silk protein MaSPII and an artificial elastin which is mainly observed in tubers.
Female golden silk orbweaver spiders Nephila clavipes incorporate MaSpII protein within the very strong and elastic silk of the main threads of the spider net which is of high industrial interest.
The artificial elastin 100xELP has high similarity to human elastin and consists of oligomeric repeats of the pentapaptide Val-Pro-Gly-Xaa-Gly (Xaa – any amino acid but proline). 100xELP exhibits a good ability to be solved in aqueous solution depending on salt concentration and temperature. It can undergo an inverse transition, i.e. the protein is soluble in an aqueous solution below its transition temperature but insoluble above this critical value.
This characteristic is delegated to its fusion partner MaSpII resulting in easy isolation and purification of spider silk protein from the biomass of potato tubers.
EN
Female golden silk orbweaver spiders Nephila clavipes incorporate MaSpII protein within the very strong and elastic silk of the main threads of the spider net which is of high industrial interest.
The artificial elastin 100xELP has high similarity to human elastin and consists of oligomeric repeats of the pentapaptide Val-Pro-Gly-Xaa-Gly (Xaa – any amino acid but proline). 100xELP exhibits a good ability to be solved in aqueous solution depending on salt concentration and temperature. It can undergo an inverse transition, i.e. the protein is soluble in an aqueous solution below its transition temperature but insoluble above this critical value.
This characteristic is delegated to its fusion partner MaSpII resulting in easy isolation and purification of spider silk protein from the biomass of potato tubers.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-12106-6 Organism Solanum tuberosum (Potato, SOLTU)Crops
Cultivar/breeding line: Albatros
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Potato modified to synthesise fusion spider silk/ elastin protein| Resistance to antibiotics (Kanamycin), Use in industrial applications
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Potato modified to synthesise fusion spider silk fragment/ elastin protein| Resistance to antibiotics (Kanamycin), Use in industrial applications
pCB301-Kan-MaSpII-100xELP, a derivat of the binary vector pCB301-Kan
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- Agrobacterium-mediated DNA transfer
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)Promoter
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BCH-GENE-SCBD-103025-2 LeB4 Signal peptide | Vicia faba (Broad Bean, Tick Bean, Windsor Bean, Horse Bean, Pigeon Bean, Field Bean)Transit signal
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BCH-GENE-SCBD-48456-2 Major Spidroin II protein coding sequence | Nephila clavipes (Golden silk orbweaver spider)Protein coding sequence | Use in industrial applications
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BCH-GENE-SCBD-103554-2 c-myc-tag | Homo sapiens (HUMAN)Transit signal
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BCH-GENE-SCBD-103553-2 Synthetic elastin-like proteinProtein coding sequence | Production of medical or pharmaceutical compounds (human or animal),Use in industrial applications
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BCH-GENE-SCBD-103023-2 KDEL ER retention signalTransit signal
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BCH-GENE-SCBD-100290-6 CaMV 35S terminator | Cauliflower mosaic virus (CaMV)Terminator
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BCH-GENE-SCBD-100270-6 Nopaline Synthase Gene Promoter | Agrobacterium tumefaciens (Agrobacterium)Promoter
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BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)Protein coding sequence | Resistance to antibiotics (Kanamycin)
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BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminator
Part of the multiple cloning site of the plasmid pBluescriptII and approximately 160 nucleotides of the right and 140 nucleotides of the left boarder sequence of the T-DNA are inserted in the potato genome but not functional in plants.
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- Research
PCR-based methods, to be developed on information about the transformation vector.
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- Purification of recombinant proteins by fusion with thermally-responsive polypeptides [ English ]
- Production of spider silk proteins in tobacco and potato.pdf [ English ]
- Production of spider silk proteins in tobacco and potato.pdf [ German ]
- Purification of Spider Silk-elastin from Transgenic Plants and Application for Human Chondrocyte Proliferation [ English ]
- A mini binary vector series for plant transformation [ English ]
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