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Living Modified Organism (LMO)
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Moonpearl carnation
EN
25947
Yes
IFD-25947-1
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Person:Dr Yukihisa KatsumotoPrincipal Researcher, Research Institute1-1-1 Wakayamadai, Shimamoto-choMishima-gun, Osaka
618-8503, JapanPhone: +81 75 962 9132,Fax: +81 75 962 3791,Website:Related OrganizationSuntory Holdings Limited (SHD)Private sector (business and industry)1-1-1 Wakayamadai, Shimamoto-choMishima-gun, Osaka
618-8503, JapanPhone: +81 75 962 9132,Fax: +81 75 962 3791,Website:
Moonpearl carnation has flowers with a unique violet/mauve colour due to the biosynthesis of the anthocyanin pigment delphinidin. The transgenic lines were derived from the parent cultivar which is a pink coloured carnation.
The genes introduced into the transgenic carnation lines included a functional dihydroflavonol reductase encoding gene (dfr) from petunia and a gene (hf1) encoding the enzyme flavonoid 3', 5'-hydroxylase (F3'5'H). Expression of the F3'5'H encoding gene allows for the production of blue coloured delphinidin anthocyanin pigments, which are not normally found in carnations.
A Dihydroflavonol-4-reductase sequence from carnation has been utilised in a dsDFR format in order to suppress expression of the endogenous carnation DFR gene, thus allowing dominant expression of the introduced petunia Dihydroflavonol-4-reductase.
EN
The genes introduced into the transgenic carnation lines included a functional dihydroflavonol reductase encoding gene (dfr) from petunia and a gene (hf1) encoding the enzyme flavonoid 3', 5'-hydroxylase (F3'5'H). Expression of the F3'5'H encoding gene allows for the production of blue coloured delphinidin anthocyanin pigments, which are not normally found in carnations.
A Dihydroflavonol-4-reductase sequence from carnation has been utilised in a dsDFR format in order to suppress expression of the endogenous carnation DFR gene, thus allowing dominant expression of the introduced petunia Dihydroflavonol-4-reductase.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-4954-7 Organism Dianthus caryophyllus (Carnation, DIACA)Crops
EN
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IFD-25958-3 - Moonberry carnation| Suntory Holdings Limited(SHD) | Changes in quality and/or metabolite content (Flavonoids (e.g. anthocyanin), Pigmentation / Coloration), Resistance to herbicides (Chlorsulfuron, Sulfonylurea)
pCGP3366
EN
- Agrobacterium-mediated DNA transfer
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-43793-4 Flavonoid 3', 5'-hydroxylase gene | Viola sp. (Pansy, VIOLA)Protein coding sequence | Changes in quality and/or metabolite content (Pigmentation / Coloration)
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BCH-GENE-SCBD-15177-7 Acetohydroxy acid synthase gene | Nicotiana tabacum (Tobacco, TOBAC )Protein coding sequence | Resistance to herbicides (Chlorsulfuron, Sulfonylurea)
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BCH-GENE-SCBD-15009-4 Dihydroflavonol-4-reductase | Petunia hybrida (Petunia, PETHY)Protein coding sequence | Changes in quality and/or metabolite content (Pigmentation / Coloration)
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BCH-GENE-SCBD-104594-4 Dihydroflavonol-4-reductase | Dianthus caryophyllus (Carnation, DIACA)Protein coding sequence | Changes in quality and/or metabolite content (Pigmentation / Coloration)
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BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)Promoter
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BCH-GENE-SCBD-101901-3 5' untranslated leader of chlorophyll a/b-binding protein | Petunia hybrida (Petunia, PETHY)Leader
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BCH-GENE-SCBD-100390-7 Acetohydroxy acid synthase gene terminator | Nicotiana tabacum (Tobacco, TOBAC )Terminator
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BCH-GENE-SCBD-103771-1 Chalcone synthase gene promoter | Antirrhinum majus (Common Snapdragon, Snapdragon)Promoter
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BCH-GENE-SCBD-103772-2 D8 gene terminator | Petunia hybrida (Petunia, PETHY)Terminator
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BCH-GENE-SCBD-105798-1 Dihydroflavonol-4-reductase promoter | Petunia hybrida (Petunia, PETHY)Promoter
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BCH-GENE-SCBD-105799-1 Dihydroflavonol-4-reductase terminator | Petunia hybrida (Petunia, PETHY)Terminator
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BCH-GENE-SCBD-105819-1 Dihydroflavonol-4-reductase intron | Petunia hybrida (Petunia, PETHY)Intron
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BCH-GENE-SCBD-100290-6 CaMV 35S terminator | Cauliflower mosaic virus (CaMV)Terminator
The dihydroflavonol-4-reductase sequence from carnation is inserted in a sense/ antisense orientation. The transcription product results in the formation of a dsDFR which suppress the expression of the endogenous DFR gene, thus allowing dominant expression of the introduced petunia Dihydroflavonol-4-reductase thus leading to the synthesis of delphinidin imparting a violet/mauve colour to the carnation.
The T-DNA is present at one integration locus and contains one copy of each T-DNA component as determined by Southern blot analysis
EN
The T-DNA is present at one integration locus and contains one copy of each T-DNA component as determined by Southern blot analysis
EN
- Ornamental
EN
Moonpearl was developed using recombinant DNA techniques to produce flowers with a unique violet/mauve colour by introducing three genes that function together in the biosynthesis of the anthocyanin pigment delphinidin.
The transgenic lines were derived from the parent cultivar which is a pink coloured carnation.The genes introduced into the transgenic carnation lines included a functional dihydroflavonol reductase encoding gene (dfr) and a gene (hf1) encoding the enzyme flavonoid 3', 5'-hydroxylase (F3'5'H), a member of the NADPH-Cytochrome P450 reductase family.
Expression of the F3'5'H encoding gene allows for the production of blue coloured delphinidin anthocyanin pigments, which are not normally found in carnations. Expression of a dsDFR construct suppresses the activity of endogenous DFR.
EN
The transgenic lines were derived from the parent cultivar which is a pink coloured carnation.The genes introduced into the transgenic carnation lines included a functional dihydroflavonol reductase encoding gene (dfr) and a gene (hf1) encoding the enzyme flavonoid 3', 5'-hydroxylase (F3'5'H), a member of the NADPH-Cytochrome P450 reductase family.
Expression of the F3'5'H encoding gene allows for the production of blue coloured delphinidin anthocyanin pigments, which are not normally found in carnations. Expression of a dsDFR construct suppresses the activity of endogenous DFR.
- IFD-25947-1 - ISAAA [ English ]
- IFD-25947-1 - SNIF [ English ]
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