IFD-264Ø7-2 - Moonvelvet carnation | BCH-LMO-SCBD-104609 | Living Modified Organism | Biosafety Clearing-House


Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
published: 14 Jan 2013 last updated: 28 Aug 2014
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Moonvelvet carnation
Moonvelvet is a flower colour modified carnation variety produced through introduction of the anthocyanin biosynthetic gene flavonoid 3',5'hydroxylase (F3'5'H) from Petunia hybrida to result in a violet/mauve colouration and the cytb5 gene to enhance the activity of flavonoid 3',5'-hydroxylase. A variant form of acetohydroxy acid/ acetolactate synthase (ALS) from Nicotiana tabacum was used as a selectable marker to confer tolerance to sulfonylurea herbicide.

Flowers of the transgenic plant produce delphinidin-related anthocyanins, not found in nature in carnation.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
Characteristics of the modification process
pCGP 2355
  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-15177-7 Acetohydroxy acid synthase gene | Nicotiana tabacum (Tobacco, TOBAC )
    Protein coding sequence | Resistance to herbicides (Chlorsulfuron, Sulfonylurea)
  • BCH-GENE-SCBD-15010-3 Flavonoid 3', 5' hydroxylase gene | Petunia hybrida (Petunia, PETHY)
    Protein coding sequence | Changes in quality and/or metabolite content (Pigmentation / Coloration)
  • BCH-GENE-SCBD-104593-1 Cytochrome b5 | Petunia hybrida (Petunia, PETHY)
    Protein coding sequence | Changes in quality and/or metabolite content (Antioxidants, Flavonoids (e.g. anthocyanin), Pigmentation / Coloration)
  • BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)
  • BCH-GENE-SCBD-101901-3 5' untranslated leader of chlorophyll a/b-binding protein | Petunia hybrida (Petunia, PETHY)
  • BCH-GENE-SCBD-100390-7 Acetohydroxy acid synthase gene terminator | Nicotiana tabacum (Tobacco, TOBAC )
  • BCH-GENE-SCBD-103771-1 Chalcone synthase gene promoter | Antirrhinum majus (Common Snapdragon, Snapdragon)
  • BCH-GENE-SCBD-103772-2 D8 gene terminator | Petunia hybrida (Petunia, PETHY)
  • BCH-GENE-SCBD-105427-1 Anthocyanidin synthase gene promoter | Dianthus caryophyllus (Carnation, DIACA)
  • BCH-GENE-SCBD-105424-1 Anthocyanidin synthase gene terminator | Dianthus caryophyllus (Carnation, DIACA)
The T-DNA is present at one integration locus and contains one copy of each T-DNA component as determined by Southern blot analysis
LMO characteristics
  • Ornamental
Detection method(s)
Additional Information
Records referencing this document Show in search
Record type Field Record(s)
Risk Assessment generated by a regulatory process Living modified organism(s) 7
Country's Decision or any other Communication Living modified organism(s) 5