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Living Modified Organism
(LMO)
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Moontea™ carnation
EN
27531
Yes
SHD-27531-4
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Person:Dr Yukihisa KatsumotoPrincipal Researcher, Research Institute1-1-1 Wakayamadai, Shimamoto-choMishima-gun, Osaka
618-8503, JapanPhone: +81 75 962 9132,Fax: +81 75 962 3791,Website:Related OrganizationSuntory Holdings Limited (SHD)Private sector (business and industry)1-1-1 Wakayamadai, Shimamoto-choMishima-gun, Osaka
618-8503, JapanPhone: +81 75 962 9132,Fax: +81 75 962 3791,Website:
Carnation variety 27531 flowers have a mauve colour due to the biosynthesis of the anthocyanin pigment delphinidin. This pigment is not produced in non-transgenic carnation. The transgenic lines were derived from the parent cultivar which is a cream coloured carnation. The genes introduced into the transgenic carnation lines included a functional dihydroflavonol reductase encoding gene (dfr) from petunia and a gene encoding the enzyme flavonoid 3', 5'-hydroxylase (F3'5'H). Expression of the F3'5'H encoding gene allows for the production of coloured delphinidin anthocyanin pigments. The modified flowers additionally contain tobacco acetolactate synthase for chlorsulfuron herbicide tolerance.
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-4954-7 Organism Dianthus caryophyllus (Carnation, DIACA)Crops
EN
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FLO-11363-2 - Moonshadow™ carnation| SUntory Holdings Ltd. | Changes in quality and/or metabolite content (Pigmentation / Coloration), Resistance to herbicides (Sulfonylurea)
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FLO-4Ø689-6 - Moonaqua™ carnation| Suntory Holdings Ltd. | Changes in quality and/or metabolite content (Pigmentation / Coloration), Resistance to herbicides (Sulfonylurea)
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FLO-4Ø685-2 - Moonvista™ carnation| SUntory Holdings Ltd. | Changes in quality and/or metabolite content (Pigmentation / Coloration), Resistance to herbicides (Sulfonylurea)
pCGP1991
EN
- Agrobacterium-mediated DNA transfer
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0.200 kb
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0.060 kb
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3.770 kb
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0.000 kb
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0.000 kb
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4.960 kb
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0.000 kb
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1.170 kb
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1.800 kb
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0.810 kb
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-15177-7 Acetohydroxy acid synthase gene | Nicotiana tabacum (Tobacco, TOBAC )Protein coding sequence | Resistance to herbicides (Chlorsulfuron, Sulfonylurea)
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BCH-GENE-SCBD-103771-1 Chalcone synthase gene promoter | Antirrhinum majus (Common Snapdragon, Snapdragon)Promoter
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BCH-GENE-SCBD-43793-4 Flavonoid 3', 5'-hydroxylase gene | Viola sp. (Pansy, VIOLA)Protein coding sequence | Changes in quality and/or metabolite content (Pigmentation / Coloration)
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BCH-GENE-SCBD-103772-2 D8 gene terminator | Petunia hybrida (Petunia, PETHY)Terminator
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BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)Promoter
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BCH-GENE-SCBD-101901-3 5' untranslated leader of chlorophyll a/b-binding protein | Petunia hybrida (Petunia, PETHY)Leader
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BCH-GENE-SCBD-100390-7 Acetohydroxy acid synthase gene terminator | Nicotiana tabacum (Tobacco, TOBAC )Terminator
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BCH-GENE-SCBD-105798-1 Dihydroflavonol-4-reductase promoter | Petunia hybrida (Petunia, PETHY)Promoter
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BCH-GENE-SCBD-15009-4 Dihydroflavonol-4-reductase | Petunia hybrida (Petunia, PETHY)Protein coding sequence | Changes in quality and/or metabolite content (Pigmentation / Coloration)
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BCH-GENE-SCBD-105799-1 Dihydroflavonol-4-reductase terminator | Petunia hybrida (Petunia, PETHY)Terminator
Gene expression
Three gene cassettes are present: Nicotiania tabacum acetolactate synthase (ALS; acetohydroxy acid synthase), Petunia hybrida dihydroflavonol-4-reductase (DFR) and Viola sp. flavonoid3', 5'-hydroxylase (F3'5'H).
Transcription of ALS is under control of a Cauliflower mosaic virus (CaMV) 35S promoter and a N. tabacum ALS terminator. A 5' untranslated leader sequence from P. hybrida chlorophyll a/b-binding protein is also present at the 5' end of ALS, but is not expected to be translated. The leader sequence promotes high levels of transcription of ALS.
Transcription of DFR is under control of its endogenous promoter and terminator. The coding sequence contains 6 exons and 5 introns.
Transcription of F3'5'H is under control of an Antirrhinum majus chalcone synthase promoter and a P. hybrida D8 terminator.
Note:
- The size of the ALS coding sequence includes the size of the terminator (3.76 kb = size of ALS coding sequence + ALS terminator)
- The size of the DFR coding sequence represents the size of the full genomic cone (4.96 kb = DFR promoter + DFR coding sequence + DFR terminator)
- The T-DNA is present at one integration locus and contains one copy of each T-DNA component as determined by Southern blot analysis
EN
Three gene cassettes are present: Nicotiania tabacum acetolactate synthase (ALS; acetohydroxy acid synthase), Petunia hybrida dihydroflavonol-4-reductase (DFR) and Viola sp. flavonoid3', 5'-hydroxylase (F3'5'H).
Transcription of ALS is under control of a Cauliflower mosaic virus (CaMV) 35S promoter and a N. tabacum ALS terminator. A 5' untranslated leader sequence from P. hybrida chlorophyll a/b-binding protein is also present at the 5' end of ALS, but is not expected to be translated. The leader sequence promotes high levels of transcription of ALS.
Transcription of DFR is under control of its endogenous promoter and terminator. The coding sequence contains 6 exons and 5 introns.
Transcription of F3'5'H is under control of an Antirrhinum majus chalcone synthase promoter and a P. hybrida D8 terminator.
Note:
- The size of the ALS coding sequence includes the size of the terminator (3.76 kb = size of ALS coding sequence + ALS terminator)
- The size of the DFR coding sequence represents the size of the full genomic cone (4.96 kb = DFR promoter + DFR coding sequence + DFR terminator)
- The T-DNA is present at one integration locus and contains one copy of each T-DNA component as determined by Southern blot analysis
EN
- Ornamental
EN
EN
| Record type | Field | Record(s) | |
|---|---|---|---|
| Country's Decision or any other Communication | LMO identification | 1 | |
| Risk Assessment generated by a regulatory process | Living modified organism(s) | 3 | |
