HZU-HHØØ1-9 - Insect resistent rice | BCH-LMO-SCBD-106014 | Living Modified Organism | Biosafety Clearing-House

Living Modified Organism (LMO)
Decisions on the LMO Risk Assessments  
published: 30 Oct 2014 last updated: 26 Jul 2019
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Insect resistent rice
Bt63 (TT51-1)
Modified rice BT63 (TT51-1) is an insect-resistant strain that expresses a codon-optimized, truncated Cry1Ab/Ac fusion cassette derived from sequences of Bacillus thuringiensis subsp. Kurstaki HD1 and HD73. The synthetic construct encodes an insecticidal protein conferring resistance against certain lepidoptera species.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
Characteristics of the modification process
pFHBT1 and pGL2RC7
  • Biolistic / Particle gun
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-100355-6 Rice actin 1, intron | Oryza sativa (Rice, ORYSA)
  • BCH-GENE-SCBD-103109-5 Cry1Ab/Ac | Bacillus thuringiensis (Bt, Bacillus, BACTU)
    Protein coding sequence | Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths), Cotton bollworm (Helicoverpa spp.))
  • BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)
  • BCH-GENE-SCBD-14975-5 Beta-lactamase gene | Escherichia coli (ECOLX)
    Protein coding sequence | Resistance to antibiotics (Ampicillin)
  • BCH-GENE-SCBD-100364-5 Rice actin 1 gene promoter | Oryza sativa (Rice, ORYSA)
  • BCH-GENE-SCBD-14991-8 Hygromycin B phosphotransferase gene | Escherichia coli (ECOLX)
    Protein coding sequence | Resistance to antibiotics (Hygromycin),Selectable marker genes and reporter genes
The expression of the hybrid Crystal protein 1Ab/Ac (Cry1Ab/Ac) is under the control of a Oryza sativa (rice) Actin 1 gene promoter and the Agrobacterium tumefaciens nopaline synthase (nos) terminator. The first intron of rice Actin 1 was included with the promoter to promote expression of Cry1Ab/Ac in the rice plant.

Hybrid Cry1Ab/Ac:
The hybrid Cry1Ab/Ac consists of 1344 bp from B. thuringiensis HD1 Cry1Ab and 486 bp from B. thuringiensis HD73 Cry1Ac. The sequence of the hybrid Cry1Ab/Ac was sequence optimized for high G/C content for efficient expression in rice.

Information regarding the transgene insertion:
The transgenic insert contains two tandem repeats of a Cry1Ab/Ac cassette integrated at the same site as a head-to tail concatemer. The first Cry1Ab/Ac cassette at the 5' end of the transgenic insert contains a truncated Actin 1 promoter (149 bp) which is missing the regulatory elements for maximal gene expression and cannot encode for a functional protein.

The second Cry1Ab/Ac cassette is complete. An 83 bp long segment of the Amp gene is located between the two tandem repeats of the Cry1Ab/Ac cassette. Incomplete ampicillin (653 bp) and hygromycin (50 bp) resistance genes are further located in the 3' downstream region.
LMO characteristics
  • Food
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