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Living Modified Organism
(LMO)
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Papaya modified for resistance to Papaya Ringspot Virus
EN
X17-2
Yes
UFL-X17CP-9
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Person:Michael J. DavisProfessor, Institute of Food and Agricultural Sciences, Citrus Research and Education Center700 Experiment Station RoadLake Alfred, Florida
33850-1299, United States of AmericaPhone:Fax:Email:Website:Related OrganizationUniversity of Florida ()Academic or research institute700 Experiment Station RoadLake Alfred, Florida
33850-1299, United States of AmericaPhone:Fax:Email:Website:
Transgenic papaya modified to express the coat protein (CP) gene of Hawaiian PRSV isolate HA5-1 thereby conferring resistance to the Papaya Ringspot Virus.
NOTE: This LMO was formerly referred to with the UID UFL-X17CP-6
EN
NOTE: This LMO was formerly referred to with the UID UFL-X17CP-6
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-12085-4 Organism Carica papaya (Papaya, Pawpaw, Papaw, CARPA)Crops
EN
pBI121fs
EN
- Agrobacterium-mediated DNA transfer
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-100270-6 Nopaline Synthase Gene Promoter | Agrobacterium tumefaciens (Agrobacterium)Promoter
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BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)Protein coding sequence | Resistance to antibiotics (Kanamycin)
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BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminator
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BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)Promoter
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BCH-GENE-SCBD-15026-6 PRSV coat protein | Papaya ringspot virus (PRSV)Protein coding sequence | Resistance to diseases and pests (Papaya ringspot potyvirus resistance)
A uidA leader sequence from E.Coli is inserted after the CaMV 35S promoter to enhance the expression of PRSV CP in papaya.
The developer reports that the insert in line X17-2 has persisted as a single copy through six generations of progeny following the initial transformation and that no vector backbone sequence is present, as determined by Southern blot. The developer also notes that PCR analysis of the 5th generation progeny of the original transformant reveals the elimination of the frame shift in the prsv-cp gene, and that low level expression of PRSV-CP is detectable by Western blot (FDA).
EN
The developer reports that the insert in line X17-2 has persisted as a single copy through six generations of progeny following the initial transformation and that no vector backbone sequence is present, as determined by Southern blot. The developer also notes that PCR analysis of the 5th generation progeny of the original transformant reveals the elimination of the frame shift in the prsv-cp gene, and that low level expression of PRSV-CP is detectable by Western blot (FDA).
EN
- Food
EN
EN
- Papaya ringspot virus-resistant papaya line X17-2 - FDA [ English ]
- X17-2 - ISAAA [ English ]
- X17-2 - OECD [ English ]
