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Living Modified Organism
(LMO)
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Maize with altered growth and tolerance to herbicides
EN
Zea Mays B104(GA2ox::KLUH) x CML91
No
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Person:Dr. Hilde Nelissen, Departement PlantensysteembiologieTechnologiepark 927Gent,
B-9052, BelgiumPhone: +32 9 2446611,Fax:Email: vib@vib.be,Website: http://www.vib.be,Related OrganizationVIB - Universiteit Gent (VIB-UGent)Academic or research instituteTechnologiepark 927Gent,
B-9052, BelgiumPhone: +32 9 2446611,Fax:Email: vib@vib.be,Website: http://www.vib.be,
The genetically modified maize plants have modified growth characteristics resulting from the additional expression of the CYP78A1 gene (also called the KLUH or PLA1 gene) under the control of the GA2oxidase promoter.
The CYP78A1 gene codes for a cytochrome P450 mono-oxygenase enzyme that is involved in the production of factors that control cell proliferation. As a result of the modification the plants have a little more robust appearance, but above all significantly larger leaves. The modification also results in more branches being formed on the tassel and a shorter anthesis-silking interval. The modified maize demonstrates longer cell division intervals, which compensate for abiotic stress, such as cold and drought.
Additionally the bar-gene from Streptomyces hygroscopicus is present in the plant as a selection marker gene. This bar-gene produces the Phosphinotricin Acetyl Transferase enzyme (PAT), which acetylates phosphinotricin, also known as glufosinate, the active ingredient of herbicides such as Basta and Liberty.
EN
The CYP78A1 gene codes for a cytochrome P450 mono-oxygenase enzyme that is involved in the production of factors that control cell proliferation. As a result of the modification the plants have a little more robust appearance, but above all significantly larger leaves. The modification also results in more branches being formed on the tassel and a shorter anthesis-silking interval. The modified maize demonstrates longer cell division intervals, which compensate for abiotic stress, such as cold and drought.
Additionally the bar-gene from Streptomyces hygroscopicus is present in the plant as a selection marker gene. This bar-gene produces the Phosphinotricin Acetyl Transferase enzyme (PAT), which acetylates phosphinotricin, also known as glufosinate, the active ingredient of herbicides such as Basta and Liberty.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-246-6 Organism Zea mays (Maize, Corn, MAIZE)Crops
Cultivar/ Breeding line: inbred line B104
EN
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Maize with altered growth and tolerance to herbicides| VIB-UGent | Changes in physiology and/or production (Growth rate), Changes in quality and/or metabolite content (Cellulose, Lignin), Resistance to herbicides (Glufosinate)
pBbm42GW7
EN
- Agrobacterium-mediated DNA transfer
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0.270 kb
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0.550 kb
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0.085 kb
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0.940 kb
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-107959-1 GA2-oxidase promoter | Zea mays (Maize, Corn, MAIZE)Promoter
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BCH-GENE-SCBD-100290-6 CaMV 35S terminator | Cauliflower mosaic virus (CaMV)Terminator
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BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminator
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BCH-GENE-SCBD-14972-12 Phosphinothricin N-acetyltransferase gene | Streptomyces hygroscopicus (STRHY)Protein coding sequence | Resistance to herbicides (Glufosinate)
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BCH-GENE-SCBD-104820-3 Omega 5' untranslated leader | Tobacco mosaic virus (TMV)Leader
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BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)Promoter
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BCH-GENE-SCBD-107955-2 Plastochron 1 | Zea mays (Maize, Corn, MAIZE)Protein coding sequence | Changes in physiology and/or production (Growth rate, Reproduction, Yield),Tolerance to abiotic stress (Cold / Heat, Drought)
Transcription of the Zea mays (maize) plastochron1 (ZmPla1) gene commences from the maize GA2-oxidase (GA2ox) promoter and terminates at the Cauliflower Mosaic Virus (CaMV) 35S terminator. The transcribed sequence of ZmPla1 was derived from the reverse transcription of the messenger RNA for ZmPLA1 (cDNA). Thus, no introns are present in the coding sequence that was integrated into the genome.
In the other gene cassette, transcription of the Streptomyces hygroscopicus phosphinothricin N-acetyltransferase (bar) gene starts from the CaMV 35S promoter and ends at the Agrobacterium tumefaciens nopaline synthase terminator. The transcript contains a 5' untranslated Tobacco Mosaic Virus omega leader sequence to enhance translation of the bar gene.
EN
In the other gene cassette, transcription of the Streptomyces hygroscopicus phosphinothricin N-acetyltransferase (bar) gene starts from the CaMV 35S promoter and ends at the Agrobacterium tumefaciens nopaline synthase terminator. The transcript contains a 5' untranslated Tobacco Mosaic Virus omega leader sequence to enhance translation of the bar gene.
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BCH-GENE-SCBD-107955-2 Plastochron 1 | Zea mays (Maize, Corn, MAIZE)Protein coding sequence | Changes in physiology and/or production (Growth rate, Reproduction, Yield),Tolerance to abiotic stress (Cold / Heat, Drought)
The expression of the ZmPla1 occurs from two sources: the native, endogenous gene and the integrated gene cassette. Thus, it is expected that an elevated level of transcript and protein would result compared to a non-modified maize.
EN
- Food
- Feed
- Biofuel
EN
EN
- Control of Plant Organ Size by KLUH/CYP78A5-Dependent Intercellular Signaling [ English ]
- Genome-wide identification of gibberellins metabolic enzyme genes and expression profiling analysis during seed germination in maize [ English ]
- Altered expression of maize PLASTOCHRON1 enhances biomass and seed yield by extending cell division duration.pdf [ English ]
| Record type | Field | Record(s) | |
|---|---|---|---|
| Risk Assessment generated by a regulatory process | Living modified organism(s) | 2 | |
| Living Modified Organism | Recipient Organism or Parental Organisms | 1 | |
| Country's Decision or any other Communication | LMO identification | 1 | |
