Potato modified for Potato Leaf Roll Virus resistance | BCH-LMO-SCBD-111084 | Living Modified Organism | Biosafety Clearing-House
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Living Modified Organism (LMO)
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Decisions on the LMO Risk Assessments  
last updated: 26 Oct 2016
Living Modified Organism identity
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Potato modified for Potato Leaf Roll Virus resistance
EN
VR/T18; VR/T21; VR/T23
No
Potato plants were modified with the insertion of a consitiutively expressed pr17 gene of the potato leafroll virus, which is presumed to be a transport protein which spreads the viral genome as a ribonucleoprotein complex by the passage through plasmodesmata in the phloem of infected plants.

As a result of the genetic modification, in the event of a PLRV infection, the modified protein could inhibit the formation of the viral transport structures in the genetically modified plants by forming hetero-oligomers with the wild-type transport protein encoded by the viral genome.

This could prevent the long-distance transport of viral RNA in the phloem and consequently the spread and further multiplication of the virus in the plant. Greenhouse experiments revealed that the genetically modified plants were resistant to the potato viruses PLRV, PVX and PVY.
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
EN
Characteristics of the modification process
Derivative of pBIN19
EN
  • Agrobacterium-mediated DNA transfer
 
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)
    Promoter
  • BCH-GENE-SCBD-111083-1 Protein 17 coding sequence | Potato leafroll virus (PLRV)
    Protein coding sequence | Resistance to diseases and pests (Viruses, Potato leaf roll virus (PLRV), Potato virus Y (PVY))
  • BCH-GENE-SCBD-100290-6 CaMV 35S terminator | Cauliflower mosaic virus (CaMV)
    Terminator
  • BCH-GENE-SCBD-100270-6 Nopaline Synthase Gene Promoter | Agrobacterium tumefaciens (Agrobacterium)
    Promoter
  • BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)
    Protein coding sequence | Resistance to antibiotics (Kanamycin)
  • BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)
    Terminator
The PLRV gene coding for the pr17 protein was modified by the addition of a 5' terminal extension by fusion to the multiple cloning site (polylinker) of the vector pBluescript. By targeted mutagenesis, the first two AUG translation start codons of the pr17 gene were changed into ACG codons and an AUG codon was inserted into the polylinker sequence.
EN
LMO characteristics
EN
  • Research
Detection method(s)
EN
Additional Information
EN
Records referencing this document Show in search
Record type Field Record(s)
Country's Decision or any other Communication Living modified organism(s) 1
Risk Assessment generated by a regulatory process Living modified organism(s) 1

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