Herbicide tolerant Maize | BCH-LMO-SCBD-112264 | Living Modified Organism | Biosafety Clearing-House

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Living Modified Organism (LMO)
  |  
Decisions on the LMO Risk Assessments  
last updated: 17 Jul 2017
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Herbicide tolerant Maize
EN
HCEM485
No
HCEM485 maize line was modified for glyphosate herbicide tolerance by inserting a 6.0 kb maize genomic fragment containing a modified, glyphosate tolerant form of the endogenous maize EPSPS encoding gene.
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
Parent line: Stine 963
EN
Characteristics of the modification process
pHCEM
EN
  • Direct DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
The maize EPSPS coding sequence was specifically modified by site-directed mutagenesis to introduce two single-nucleotide substitutions. These two point mutations resulted in a codon change from threonine to isoleucine  at position 102 (relative to the amino acid sequence of the native maize EPSPS enzyme) and
a proline to serine change at position 106.

Molecular characterization of the insertion suggests that 4 complete copies of the fragment are inserted at a single location.
EN
LMO characteristics
EN
  • Food
  • Feed
Detection method(s)
EN
Additional Information
EN
Records referencing this document Show in search
Record type Field Record(s)
Country's Decision or any other Communication Living modified organism(s) 1
Risk Assessment generated by a regulatory process Living modified organism(s) 1