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Living Modified Organism (LMO)
  |  
Decisions on the LMO Risk Assessments  
published: 01 Feb 2019 last updated: 06 Feb 2019
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Insect resistant, Herbicide tolerant Maize
EN
MON87427 × MON89034 × TC1507 × MON88017
Yes
MON-87427-7 × MON-89Ø34-3 × DAS-Ø15Ø7-1 × MON-88Ø17-3
The stacked maize line was obtained through the traditional cross breeding of the parental organisms MON87427, MON89034, MON88017, and DAS01507. The line inherited genes that express cry1A.105, cry2Ab2,  cry3Bb1, cry1F, pat, and cp4epsps. The expression of these genes confer phosphinothricin (PPT), specifically glufosinate ammonium, and glyphosate herbicide tolerance, as well as resistance to Lepidoptera and Coleoptera pests.
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
  • BCH-ORGA-SCBD-246-6 Organism Zea mays (Maize, Corn, MAIZE)
    Crops
  • BCH-LMO-SCBD-104758-3 Living Modified Organism MON-87427-7 - Maize modified for tissue selective glyphosate tolerance
    Monsanto | Resistance to herbicides (Glyphosate)
  • BCH-LMO-SCBD-43773-18 Living Modified Organism MON-89Ø34-3 - YieldGard™ VT Pro™
    Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths))
  • BCH-LMO-SCBD-14841-16 Living Modified Organism DAS-Ø15Ø7-1 - Herculex™ I maize
    Dow AgroSciences, Pioneer Hi-Bred International Inc. | Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths), European corn borer (Ostrinia nubilalis)), Resistance to herbicides (Glufosinate)
  • BCH-LMO-SCBD-15106-10 Living Modified Organism MON-88Ø17-3 - YieldGard™ VT™ Rootworm/RR2™ Maize
    Monsanto | Resistance to diseases and pests (Insects, Coleoptera (beetles)), Resistance to herbicides (Glyphosate)
EN
Characteristics of the modification process
PV-ZMAP1043, PV-ZMIR245,  PV-ZMIR39,  PHI8999A (derived from plasmid PHP8999)
EN
  • Cross breeding
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-100366-6 CaMV Enhanced 35S promoter | Cauliflower mosaic virus (CaMV)
    Promoter
  • BCH-GENE-SCBD-100359-7 Hsp70 intron | Zea mays (Maize, Corn, MAIZE)
    Intron
  • BCH-GENE-SCBD-100365-6 Chloroplast transit peptide 2 | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)
    Transit signal
  • BCH-GENE-SCBD-14979-7 5-enolpyruvylshikimate-3-phosphate synthase gene | Agrobacterium tumefaciens (Agrobacterium)
    Protein coding sequence | Resistance to herbicides (Glyphosate)
  • BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)
    Terminator
  • BCH-GENE-SCBD-43771-9 Cry1A.105 | Bacillus thuringiensis (Bt, Bacillus, BACTU)
    Protein coding sequence | Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths))
  • BCH-GENE-SCBD-100354-6 5' untranslated leader from chlorophyll a/b-binding protein | Triticum aestivum (Wheat)
    Leader sequence
  • BCH-GENE-SCBD-100355-6 Rice actin 1, intron | Oryza sativa (Rice, ORYSA)
    Intron
  • BCH-GENE-SCBD-100356-6 Heat shock protein 17.3 terminator | Triticum aestivum (Wheat)
    Terminator
  • BCH-GENE-SCBD-101507-5 FMV 34S promoter | Figwort mosaic virus (Figwort mottle virus, FMV, CMoVb)
    Promoter
  • BCH-GENE-SCBD-100360-4 Transit peptide and first intron of Rubisco SSU | Zea mays (Maize, Corn, MAIZE)
    Transit signal
  • BCH-GENE-SCBD-14988-7 Cry2Ab2 | Bacillus thuringiensis (Bt, Bacillus, BACTU)
    Protein coding sequence | Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths))
  • BCH-GENE-SCBD-101415-9 Ti plasmid left border repeat | Agrobacterium tumefaciens (Agrobacterium)
    Plasmid vector
  • BCH-GENE-SCBD-101416-7 Ti plasmid right border repeat | Agrobacterium tumefaciens (Agrobacterium)
    Plasmid vector
  • BCH-GENE-SCBD-14993-5 Cry3Bb1 | Bacillus thuringiensis (Bt, Bacillus, BACTU)
    Protein coding sequence | Resistance to diseases and pests (Insects, Coleoptera (beetles))
  • BCH-GENE-SCBD-100364-5 Rice actin 1 gene promoter | Oryza sativa (Rice, ORYSA)
    Promoter
  • BCH-GENE-SCBD-14987-8 Cry1F | Bacillus thuringiensis (Bt, Bacillus, BACTU)
    Protein coding sequence | Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths))
  • BCH-GENE-SCBD-15002-5 Phosphinothricin N-acetyltransferase | Streptomyces viridochromogenes (STRVR)
    Protein coding sequence | Resistance to herbicides (Glufosinate)
  • BCH-GENE-SCBD-100362-7 Ubiquitin gene promoter | Zea mays (Maize, Corn, MAIZE)
    Promoter
  • BCH-GENE-SCBD-100363-5 ORF25 PolyA Terminator sequence | Agrobacterium tumefaciens (Agrobacterium)
    Terminator
  • BCH-GENE-SCBD-100290-6 CaMV 35S terminator | Cauliflower mosaic virus (CaMV)
    Terminator
  • BCH-GENE-SCBD-103627-5 Ubiquitin Intron 1 | Zea mays (Maize, Corn, MAIZE)
    Intron
  • BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)
    Promoter
DNA insert from MON87427, vector PV-ZMAP1043:
MON87427 was modifies to express the CP4 EPSPS protein which confers tolerance to the herbicide glyphosate. The e35S-hsp70 promoter and intron combination is used to drive the tissue selective expression of the cp4 epsps gene resulting in CP4 EPSPS protein production in vegetative and female reproductive tissue, providing tolerance to glyphosate within these tissues.

DNA insert from MON89034 vector PV-ZMIR245:
Maize line MON89034 expresses two Bt-toxins encoded by the genes cry1A.105 and cry2Ab2 from Bacillus thuringiensis that confer resistance against certain lepidopteran pests.

DNA insert from DAS1507 vector PHI8999A:
DNA fragment PHI8999A contains two adjacent plant gene expression cassettes for cry1F, conferring resistance to European corn borer (Ostrinia nubilalis), and pat for glufosinate herbicide tolerance. The coding sequence of both genes has been optimized to achieve a high level of expression in maize. A leucine residue was modified (replacing a phenylalanine at position 604 of 605 amino acids in total) of Cry1F to create a specific restriction site for cloning purposes.

DNA insert from MON88017 vector PV-ZMIR39:
MON88017 expresses the cry3Bb1 gene encoding a Coleopteran-specific insecticidal protein to control infestation with corn root worm, and the cp4 epsps gene.

For additional information on this LMO, please refer to the records of the parental LMOs.
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LMO characteristics
EN
  • Food
  • Feed
  • Biofuel
Detection method(s)
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Additional Information
EN
Records referencing this document Show in search
Record type Field Record(s)
Country's Decision or any other Communication LMO identification 1
Risk Assessment generated by a regulatory process Living modified organism(s) 1