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Living Modified Organism
(LMO)
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Maize with altered growth and tolerance to herbicides
EN
pUBIL-GA20OX1 × pGA2ox-PLA1
No
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Person:Dr. Hilde Nelissen, Departement PlantensysteembiologieTechnologiepark 927Gent,
B-9052,Phone: +32 9 2446611,Fax:Email: vib@vib.be,Website: http://www.vib.be,Related OrganizationVIB - Universiteit Gent (VIB-UGent)Technologiepark 927Gent,
B-9052,Phone: +32 9 2446611,Fax:Email: vib@vib.be,Website: http://www.vib.be,
The modified maize plant was produced through crossing of two modified lines (stacked event) that demonstrate modified growth characteristics resulting from the additional expression of two genes: (1) the Arabidopsis thaliana GA20oxidase (gibberellin 2-beta-dioxygenase) under the control of a Zea mays (maize) pUBIL promoter and (2) the maize PLA1 gene (plastochron1; also known as the ‘KLUH’ gene, or CYP78A1 gene) under the control of the maize GA2oxidase promoter.
The GA20oxidase gene is involved in the production of bio-active gibberellins, which cause a cellular increase in cellulose, lignin, and cell wall residue. The PLA1 gene codes for a cytochrome P450 mono-oxygenase enzyme that is involved in the production of factors that control cell proliferation. As a result of the modification, the plants have more rapid and prolonged growth, which leads to plants that have significantly larger leaves, a higher biomass, and a significantly higher cob yield. The modification also results in more branches being formed on the tassel and a shorter anthesis-silking interval.
The plant also contains the bar gene which produces the Streptomyces hygroscopicus phosphinothricin N-acetyltransferase (PAT) protein as a selection marker gene to confer tolerance to the herbicide glufosinate.
EN
The GA20oxidase gene is involved in the production of bio-active gibberellins, which cause a cellular increase in cellulose, lignin, and cell wall residue. The PLA1 gene codes for a cytochrome P450 mono-oxygenase enzyme that is involved in the production of factors that control cell proliferation. As a result of the modification, the plants have more rapid and prolonged growth, which leads to plants that have significantly larger leaves, a higher biomass, and a significantly higher cob yield. The modification also results in more branches being formed on the tassel and a shorter anthesis-silking interval.
The plant also contains the bar gene which produces the Streptomyces hygroscopicus phosphinothricin N-acetyltransferase (PAT) protein as a selection marker gene to confer tolerance to the herbicide glufosinate.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-LMO-SCBD-107960-4 Living Modified Organism Maize with altered growth and tolerance to herbicidesVIB - Universiteit Gent(VIB-UGent) | Changes in physiology and/or production (Growth rate, Reproduction, Yield), Resistance to herbicides (Glufosinate), Tolerance to abiotic stress (Cold / Heat, Drought)
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BCH-LMO-SCBD-103523-6 Living Modified Organism Maize with altered growth and tolerance to herbicidesVIB-UGent | Changes in physiology and/or production (Growth rate), Changes in quality and/or metabolite content (Cellulose, Lignin), Resistance to herbicides (Glufosinate)
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BCH-ORGA-SCBD-246-6 Organism Zea mays (Maize, Corn, MAIZE)Crops
Cultivar/ Breeding line: inbred line B104
EN
pMBb7Fm21GW-UBIL; pBbm42GW7
EN
- Cross breeding
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0.550 kb
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-100362-7 Ubiquitin gene promoter | Zea mays (Maize, Corn, MAIZE)Promoter
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BCH-GENE-SCBD-111070-1 Gibberellin 2-beta-dioxygenase gene | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)Protein coding sequence | Changes in physiology and/or production (Growth rate)
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BCH-GENE-SCBD-100290-6 CaMV 35S terminator | Cauliflower mosaic virus (CaMV)Terminator
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BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)Promoter
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BCH-GENE-SCBD-14972-12 Phosphinothricin N-acetyltransferase gene | Streptomyces hygroscopicus (STRHY)Protein coding sequence | Resistance to herbicides (Glufosinate)
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BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminator
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BCH-GENE-SCBD-104820-3 Omega 5' untranslated leader | Tobacco mosaic virus (TMV)Leader
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BCH-GENE-SCBD-107959-1 GA2-oxidase promoter | Zea mays (Maize, Corn, MAIZE)Promoter
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BCH-GENE-SCBD-107955-2 Plastochron 1 | Zea mays (Maize, Corn, MAIZE)Protein coding sequence | Changes in physiology and/or production (Growth rate, Reproduction, Yield),Tolerance to abiotic stress (Cold / Heat, Drought)
Genetic elements associated with pMBb7Fm21GW-UBIL:
Transcription of the Arabidopsis thaliana gibberellin 2-beta-dioxygenase (GA20 oxidase) is under control of the Zea mays (maize) ubiquitin gene promoter and the Cauliflower Mosaic Virus (CaMV) 35S terminator.
Transcription of the herbicide tolerance gene phosphinothricin N-acetyltransferase (bar; pat) from Streptomyces hygroscopus commences from the CaMV 35S promoter and ends at the Agrobacterium tumefaciens nopaline synthase terminator. The transcript contains a Tobacco Mosaic Virus 5' untranslated omega leader sequence that promotes the translation of PAT.
Genetic elements associated with pBbm42GW7:
Transcription of the maize plastochron1 (ZmPla1) gene commences from the maize GA2-oxidase (GA2ox) promoter and terminates at the Cauliflower Mosaic Virus (CaMV) 35S terminator. The transcribed sequence of ZmPla1 was derived from the reverse transcription of the messenger RNA for ZmPLA1 (cDNA). Thus, no introns are present in the coding sequence that was integrated into the genome.
In the other gene cassette, transcription of bar starts from the CaMV 35S promoter and ends at the A. tumefaciens nopaline synthase terminator. The transcript contains a 5' untranslated Tobacco Mosaic Virus omega leader sequence to enhance translation of the bar gene.
Kindly refer to the parental records for more information.
EN
Transcription of the Arabidopsis thaliana gibberellin 2-beta-dioxygenase (GA20 oxidase) is under control of the Zea mays (maize) ubiquitin gene promoter and the Cauliflower Mosaic Virus (CaMV) 35S terminator.
Transcription of the herbicide tolerance gene phosphinothricin N-acetyltransferase (bar; pat) from Streptomyces hygroscopus commences from the CaMV 35S promoter and ends at the Agrobacterium tumefaciens nopaline synthase terminator. The transcript contains a Tobacco Mosaic Virus 5' untranslated omega leader sequence that promotes the translation of PAT.
Genetic elements associated with pBbm42GW7:
Transcription of the maize plastochron1 (ZmPla1) gene commences from the maize GA2-oxidase (GA2ox) promoter and terminates at the Cauliflower Mosaic Virus (CaMV) 35S terminator. The transcribed sequence of ZmPla1 was derived from the reverse transcription of the messenger RNA for ZmPLA1 (cDNA). Thus, no introns are present in the coding sequence that was integrated into the genome.
In the other gene cassette, transcription of bar starts from the CaMV 35S promoter and ends at the A. tumefaciens nopaline synthase terminator. The transcript contains a 5' untranslated Tobacco Mosaic Virus omega leader sequence to enhance translation of the bar gene.
Kindly refer to the parental records for more information.
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BCH-GENE-SCBD-107955-2 Plastochron 1 | Zea mays (Maize, Corn, MAIZE)Protein coding sequence | Changes in physiology and/or production (Growth rate, Reproduction, Yield),Tolerance to abiotic stress (Cold / Heat, Drought)
The expression of the ZmPla1 occurs from two sources: the native, endogenous gene and the integrated gene cassette. Thus, it is expected that an elevated level of transcript and protein would result compared to a non-modified maize.
EN
- Biofuel
- Feed
- Food
- Research
EN
EN
