Maltose binding protein affinity tag | Escherichia coli (ECOLX) | BCH-GENE-SCBD-115695 | Genetic element | Biosafety Clearing-House

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Genetic element (GENE)

last updated: 01 Sep 2020
General information
Maltose binding protein affinity tag
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MBP tag
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Protein coding sequence
No
Donor organism
Sequence sourced from the malE gene in E. coli.
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Characteristics of the protein coding sequence
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The maltose binding protein (MBP) purification tag is an amino acid sequence attached proteins to allow for the purification and isolation of a recombinant using amylose resin-based chromatography. The methodology exploits the maltose binding capabilities of Escherichia coli gene malE (maltose/maltodextrin-binding periplasmic protein). During purification, the MBP tag binds to amylose resin while the other, non-tagged proteins are eluted. Next, malltose is added to release the MBP-tagged protein from the resin.

The MPB tag improves solubility and reduces toxicity of the recombinant protein compared to other affinity tags. However, the MBP tag is larger than other affinity tags and can potentially be more allergenic.
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