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Genetic element
(GENE)
last updated: 01 Sep 2020
Maltose binding protein affinity tag
EN
MBP tag
EN
Protein coding sequence
No
-
BCH-ORGA-SCBD-14930-5 Organism Escherichia coli (ECOLX)Bacteria
Sequence sourced from the malE gene in E. coli.
EN
EN
The maltose binding protein (MBP) purification tag is an amino acid sequence attached proteins to allow for the purification and isolation of a recombinant using amylose resin-based chromatography. The methodology exploits the maltose binding capabilities of Escherichia coli gene malE (maltose/maltodextrin-binding periplasmic protein). During purification, the MBP tag binds to amylose resin while the other, non-tagged proteins are eluted. Next, malltose is added to release the MBP-tagged protein from the resin.
The MPB tag improves solubility and reduces toxicity of the recombinant protein compared to other affinity tags. However, the MBP tag is larger than other affinity tags and can potentially be more allergenic.
EN
The MPB tag improves solubility and reduces toxicity of the recombinant protein compared to other affinity tags. However, the MBP tag is larger than other affinity tags and can potentially be more allergenic.
EN
- Several Affinity Tags Commonly Used in Chromatographic Purification.pdf [ English ]
- Maltose-Binding Protein (MBP), a Secretion-Enhancing Tag for Mammalian Protein Expression Systems.pdf [ English ]
- A generic protocol for the expression and purification of recombinant proteins in Escherichia coli using a combinatorial His6-maltose binding protein fusion tag.pdf [ English ]
- Affinity Purification of a Recombinant Protein Expressed as a Fusion with the Maltose-Binding Protein (MBP) Tag.pdf [ English ]
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