The Indian mustard (Brassica juncea) was modified for restoring male fertility and herbicide tolerance. For male fertility restoration, the mustard expresses Bacillus amyloliquefaciens barstar, a protein inhibitor of barnase, in the tapetum cell layer of the pollen sac during anther development. Barnase is an RNase, which degrades RNA in the pollen and prevents the pollen from developing. With barstar expression, the RNase is inhibited and the pollen develops normally. Thus, crossing barstar expressing lines with barnase expressing (male sterile) lines restores male fertility in the progeny.

For glufosinate tolerance, the mustard expresses Streptomyces hygroscopicus phosphinothricin N-acetyltransferase, which inactivates the herbicide through acetylation.

To achieve the fertility restorer line EH-2 modbs 2.99 for hybrid seed production, the mustard variety Varuna was initially transformed with transgenic construct and then backcrossed into variety EH-2. Six backcrossings were performed to try to remove integration of vector backbone sequence.