ACS-BNØ11-5 - Navigator™ canola | BCH-LMO-SCBD-14752 | Living Modified Organism | Biosafety Clearing-House


Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
published: 05 Jun 2006 last updated: 21 Feb 2018
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Navigator™ canola
Line Oxy-235 was developed to allow the use of oxynil herbicides, ioxynil and bromoxynil, as weed control options in canola. Canola tolerant to the oxynil herbicides created through insertion of the bxn gene isolated from the bacterium Klebsiella ozaenae, encoding a nitrilase enzyme that hydrolyzes oxynil herbicides to non-phytotoxic compounds. Bromoxynil is very effective on broadleaf weeds that are common in canola fields and the deployment of transgenic bromoxynil-tolerant canola will allow the post-emergence control of these weeds without crop injury.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
Canola variety Westar
Characteristics of the modification process
  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-14976-5 Bromoxynil-specific nitrilase | Klebsiella pneumoniae subsp. ozaenae (Klebsiella, KLEPO)
    Protein coding sequence | Resistance to herbicides (Bromoxynil)
  • BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)
  • BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)
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    BCH-GENE-SCBD-103768-2 RuBisCO small subunit gene 5'UTR | Zea mays (Maize, Corn, MAIZE)
Transgenic Oxy-235 contains a single copy of the bxn gene isolated from the bacterium Klebsiella pneumoniae (subsp. ozaenae), which encodes a nitrilase enzyme that hydrolyzes oxynil herbicides to non-phytotoxic compounds. Constitutive expression of the bxn gene was regulated via the 35S promoter from cauliflower mosaic virus (CaMV) and levels of nitrilase enzyme were detected in samples of leaf (1000 ng/mg total protein) and seed (<10 ng/mg total protein) tissue. No nitrilase was detectable in samples of refined oil (detection limit 20 ppb), and it was concluded that there will be no human exposure to this proteins.
LMO characteristics
Detection method(s)
Additional Information