DAS-21Ø23-5 x DAS-24236-5 x MON-Ø1445-2 was produced by crossing WideStrike™ insect resistant cotton (DAS-21Ø23-5 x DAS-24236-5) with the herbicide-tolerant cotton line MON-Ø1445-2. This stacked cotton line is a product of traditional plant breeding, and therefore is not automatically subject to regulation in all jurisdictions as are transgenic plants resulting from recombinant DNA technologies. Certain jurisdictions may request notification in advance of the release of a stacked hybrid, or may request information to conduct an environmental and food safety assessment.

This stacked cotton line expresses four novel proteins: the delta-endotoxins Cry1F and Cry1Ac, which confer resistance to the lepidopteran pests of cotton, such as the cotton bollworm, pink bollworm and tobacco budworm, the CP4 EPSPS protein, which confers tolerance to the herbicide glyphosate, and the PAT protein, which confers tolerance to glufosinate ammonium. The insecticidal proteins Cry1F and Cry1Ac are produced by the cry1F gene and cry1Ac genes respectively, both of which are from WideStrike™. The cotton line WideStrike™ was produced by crossing line 281-24-236 with line 3006-210-23. The PAT protein in WideStrike™ is produced by the pat gene: this expressed novel protein was intended solely for use as a selectable marker during plant transformation. The CP4 EPSPS protein is produced by the cp4 epsps gene from MON-Ø1445-2.