Acetohydroxy acid synthase gene (Chimeric) | Nicotiana tabacum (Tobacco, TOBAC ) | BCH-GENE-SCBD-15164 | Genetic element | Biosafety Clearing-House

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Genetic element (GENE)

published: 20 Jun 2006 last updated: 12 Feb 2021
General information
Acetohydroxy acid synthase gene (Chimeric)
EN
  • Acetolactate Synthase
    EN
  • CS-S4-HrA
    EN
CS-SuRB_S4-TOBAC
EN
Protein coding sequence
No
Donor organism
Nicotiana tabacum cv. Xanthi
EN
Characteristics of the protein coding sequence
Chimeric Acetohydroxy acid synthase
EN
Acetolactate synthase (ALS) catalyzes the first common step in the biosynthesis of the essential amino acids isoleucine, leucine, and valine.

Native ALS is normally inhibited by sulfonylurea herbicides. However S4-HrA is a chimeric gene derived from two different tobacco ALS genes that both encoded herbicide sensitive versions of ALS.

Two resistance mutations were introduced into one of the ALS genes (Pro191Ala and Trp568Leu) via site directed mutagenesis. The DNA fragment containing the resistance mutations was then moved into the second ALS gene through a restriction enzyme fragment.
EN
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