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Genetic element
(GENE)
last updated: 12 Feb 2021
Acetohydroxy acid synthase gene (Chimeric)
EN
-
Acetolactate SynthaseEN
-
CS-S4-HrAEN
CS-SuRB_S4-TOBAC
EN
Protein coding sequence
No
-
BCH-ORGA-SCBD-12120-4 Organism Nicotiana tabacum (Tobacco, TOBAC )Crops
Nicotiana tabacum cv. Xanthi
EN
Chimeric Acetohydroxy acid synthase
EN
Acetolactate synthase (ALS) catalyzes the first common step in the biosynthesis of the essential amino acids isoleucine, leucine, and valine.
Native ALS is normally inhibited by sulfonylurea herbicides. However S4-HrA is a chimeric gene derived from two different tobacco ALS genes that both encoded herbicide sensitive versions of ALS.
Two resistance mutations were introduced into one of the ALS genes (Pro191Ala and Trp568Leu) via site directed mutagenesis. The DNA fragment containing the resistance mutations was then moved into the second ALS gene through a restriction enzyme fragment.
EN
Native ALS is normally inhibited by sulfonylurea herbicides. However S4-HrA is a chimeric gene derived from two different tobacco ALS genes that both encoded herbicide sensitive versions of ALS.
Two resistance mutations were introduced into one of the ALS genes (Pro191Ala and Trp568Leu) via site directed mutagenesis. The DNA fragment containing the resistance mutations was then moved into the second ALS gene through a restriction enzyme fragment.
EN
- Chimeric RNA DNA Oligonucleotide-Based Site-Specific Modification of the Tobacco Acetolactate Syntase Gene.pdf [ English ]
- Isolation and characterization of plant genes coding for acetolactate synthase, the target enzyme for two classes of herbicides.pdf [ English ]
- The molecular basis of sulfonylurea herbicide resistance in tobacco.pdf [ English ]
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