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Living Modified Organism
(LMO)
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Herbicide-tolerant Indian mustard (RF3 juncea canola)
EN
RF3
No
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Melbourne,Person:BASF
, AustraliaPhone: 61 3 8855 6600,Fax:Email: reception-au@basf.com,Website:Related OrganizationBASF Australia Ltd ()
The modified Indian mustard (Brassica juncea; RF3 juncea canola) was produced via conventional crossbreeding to transfer the genetic modification from InVigor™ canola (Brassica napus; RF3) to unmodified Indian mustard. Thus, the modification is expected to confer tolerance to glufosinate herbicides through the expression of Streptomyces hygroscopicus phosphinothricin N-acetyltransferase. In addition, the modified Indian mustard contains the male fertility restoration system based on the Bacillus amyloliquefaciens barstar, which inhibits the activity of the RNase barnase.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-115905-1 Organism Brassica juncea (Indian mustard, Brown mustard, Chinese mustard, Leaf mustard, Vegetable mustard, Mustard greens, BRAJU)Crops
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BCH-LMO-SCBD-14755-9 Living Modified Organism ACS-BNØØ3-6 - InVigor™ canolaBayer CropScience | Changes in physiology and/or production (Fertility restoration), Resistance to herbicides (Glufosinate)
Line 10CJ28-094
EN
pTHW118
EN
- Cross breeding
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-14974-7 Barstar | Bacillus amyloliquefaciens (BACAM)Protein coding sequence | Changes in physiology and/or production (Fertility restoration)
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BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminator
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BCH-GENE-SCBD-103851-5 rbcS Promoter | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)Promoter
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BCH-GENE-SCBD-14972-12 Phosphinothricin N-acetyltransferase gene | Streptomyces hygroscopicus (STRHY)Protein coding sequence | Resistance to herbicides (Glufosinate)
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BCH-GENE-SCBD-101409-2 Barstar gene terminator | Bacillus amyloliquefaciens (BACAM)Terminator
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BCH-GENE-SCBD-101407-6 pTA29 pollen specific promoter | Nicotiana tabacum (Tobacco, TOBAC )Promoter
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BCH-GENE-SCBD-103067-9 Transcript 7 gene 3' untranslated region | Agrobacterium tumefaciens (Agrobacterium)Terminator
The modified Indian mustard contains two gene cassettes: Bacillus amyloliquefaciens barstar and Streptomyces hygroscopicus phosphinothricin N-acetyltransferase (bar).
The bar coding sequence is under control of Arabidopsis thaliana ribulose-1,5-bisphosphate carboxylase small subunit (rbcS) protomer and A. tumefaciens transcript 7 gene 3' untranslated region.
The barstar coding sequence is under control of the Nicotiana tabacum TA29 promoter and Agrobacterium tumefaciens nopaline synthase (nos) terminator. Despite the presence of the barstar 3' untranslated region, transcription is expected to be terminated by the nos terminator. Due to the pollen-specific nature of the promoter, expression of barstar is only expect in pollen.
A second barstar cassette is present with the same genetic elements as above. The TA29 promoter present in this second is truncated, but functional. The cassette terminated by a nos terminator. A partial rbcS promoter is also present, but not functional.
Note:
The bar coding sequence is under control of Arabidopsis thaliana ribulose-1,5-bisphosphate carboxylase small subunit (rbcS) protomer and A. tumefaciens transcript 7 gene 3' untranslated region.
The barstar coding sequence is under control of the Nicotiana tabacum TA29 promoter and Agrobacterium tumefaciens nopaline synthase (nos) terminator. Despite the presence of the barstar 3' untranslated region, transcription is expected to be terminated by the nos terminator. Due to the pollen-specific nature of the promoter, expression of barstar is only expect in pollen.
A second barstar cassette is present with the same genetic elements as above. The TA29 promoter present in this second is truncated, but functional. The cassette terminated by a nos terminator. A partial rbcS promoter is also present, but not functional.
Note:
- On the N-terminal, two codons of the wild-type bar coding region have been substituted for the codons ATG and GAC.
- Southern blot and PCR analysis indicated that the LMO contains one complete copy of the transformation cassette with a second partial cassette, arranged in an inverted repeat orientation centered around the left border. The partial repeat contains the functional genetic elements for the barstar cassette, but the rbcS promoter is non-functional.
- The exact genetic element sizes for the truncated promoters in the second partial repeat are not available and thus have not been indicated.
EN
- Feed
- Food
For more information, kindly refer to the parental LMO record.
| Record type | Field | Record(s) | |
|---|---|---|---|
| Country's Decision or any other Communication | LMO identification | 1 | |
| Risk Assessment generated by a regulatory process | Living modified organism(s) | 1 | |
