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Living Modified Organism
(LMO)
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Pod borer resistant cowpea
EN
PP099-EV113
No
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Organization:Wisconsin Crop Innovation Center (WCIC)8520 University Green, Middleton, WI 53562, United StatesMiddleton,
, United States of AmericaPhone: +1 608-262-6900,Fax:Email: arcarlson@wisc.edu,Website:
The genetically modified cowpea (Vigna unguiculata) event 'PP099-EV113' expresses the Bacillus thuringiensis Cry1Ie1 to confer resistance to lepidoptera insects, such as pod borers. The insecticidal protein has a pore forming mode-of-action (similar to other crystal delta-endotoxins), which disrupts the larval midgut epithelium and leads to death of susceptible larvae. In addition, the modified cowpea expresses Escherichia coli spectinomycin adenyltransferase, which allowed for spectinomycin selection during transformation.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-103617-3 Organism Vigna unguiculata (Cowpea, Black eyed pea)Crops
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PP099
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- Agrobacterium-mediated DNA transfer
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0.394 kb
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0.792 kb
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2.423 kb
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-103853-2 rbcS Terminator | Nicotiana tabacum (Tobacco, TOBAC )Terminator
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BCH-GENE-SCBD-277244-1 Cry1Ie1 | Bacillus thuringiensis (Bt, Bacillus, BACTU)Protein coding sequence | Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths), Cotton bollworm (Helicoverpa spp.), European corn borer (Ostrinia nubilalis), Fall armyworm (Spodoptera frugiperda))
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BCH-GENE-SCBD-277248-1 Polyubiquitin 3XL promoter | Glycine max (Soybean, Soya bean, Soya / soja /соя, SOYBN)Promoter
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BCH-GENE-SCBD-277246-1 Actin 7 terminator | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)Terminator
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BCH-GENE-SCBD-15033-8 3"(9)-O-aminoglycoside adenyltransferase | Escherichia coli (ECOLX)Protein coding sequence | Resistance to antibiotics (Streptomycin)
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BCH-GENE-SCBD-104802-5 Polyubiquitin10 gene promoter | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)Promoter
The modified cowpea contains Escherichia coli 3"(9)-O-aminoglycoside adenyltransferase (spectinomycin adenyltransferase; aadA) and Bacillus thuringiensis cry1Ie1 gene cassettes.
The aadA coding sequence is under control of a Glycine max polyubiquitin 3XL promoter and an Arabidopsis thaliana actin 7 terminator. Due to the nature of the promoter, very high levels of transcription are expected in all plant tissues.
The cry1Ie1 coding sequence is under control of an A. thaliana polyubiquitin 10 promoter and a Nicotiana tabacum ribulose-1,5-bisphosphate carboxylase small subunit terminator. Due to the nature of the promoter, high levels of transcription are expected in all plant tissues.
Note:
The aadA coding sequence is under control of a Glycine max polyubiquitin 3XL promoter and an Arabidopsis thaliana actin 7 terminator. Due to the nature of the promoter, very high levels of transcription are expected in all plant tissues.
The cry1Ie1 coding sequence is under control of an A. thaliana polyubiquitin 10 promoter and a Nicotiana tabacum ribulose-1,5-bisphosphate carboxylase small subunit terminator. Due to the nature of the promoter, high levels of transcription are expected in all plant tissues.
Note:
- The dual T-DNA binary transformation vector was constructed de novo using Golden Gate cloning, incorporating synthesised DNA fragments modified to eliminate restriction enzyme recognition sites. It includes the ColE1 origin of replication from pBR322 for growth in E. coli and the RK2 traF and oriV replication components for growth in Agrobacterium strains, as well as a bacterial neomycin phosphotransferase II selection cassette.
- The vector backbone additionally contained a GFP bacterial cassette, which was not inserted into the parental cowpea genome.
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- Food
EN
EN
| Record type | Field | Record(s) | |
|---|---|---|---|
| Risk Assessment generated by a regulatory process | Living modified organism(s) | 2 | |
| Country's Decision or any other Communication | LMO identification | 2 | |
